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dc.contributor.authorAlcolea, Pedro J
dc.contributor.authorAlonso, Ana
dc.contributor.authorGarcía-Tabares, Francisco
dc.contributor.authorToraño, Alfredo 
dc.contributor.authorLarraga, Vicente
dc.date.accessioned2019-12-02T13:17:11Z
dc.date.available2019-12-02T13:17:11Z
dc.date.issued2014
dc.identifier.citationPLoS One. 2014 Dec 11;9(12):e113837.es_ES
dc.identifier.issn1932-6203es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/8733
dc.description.abstractThe life cycle of the trypanosomatid Crithidia fasciculata is monogenetic, as the unique hosts of these parasites are different species of culicids. The comparison of these non-pathogenic microorganisms evolutionary close to other species of trypanosomatids that develop digenetic life cycles and cause chronic severe sickness to millions of people worldwide is of outstanding interest. A ground-breaking analysis of differential protein abundance in Crithidia fasciculata is reported herein. The comparison of the outcome with previous gene expression profiling studies developed in the related human pathogens of the genus Leishmania has revealed substantial differences between the motile stages of these closely related organisms in abundance of proteins involved in catabolism, redox homeostasis, intracellular signalling, and gene expression regulation. As L. major and L. infantum agglutinate with peanut lectin and non-agglutinating parasites are more infective, the agglutination properties were evaluated in C. fasciculata. The result is that choanomastigotes are able to agglutinate with peanut lectin and a non-agglutinating subpopulation can be also isolated. As a difference with L. infantum, the non-agglutinating subpopulation over-expresses the whole machinery for maintenance of redox homeostasis and the translation factors eIF5a, EF1α and EF2, what suggests a relationship between the lack of agglutination and a differentiation process.es_ES
dc.description.sponsorshipPA thanks CENTRO DE INVESTIGACIONES BIOLÓGICAS CSIC for two contracts of employment at a position included in the A1 group (respectively developed from January 16th to July 23rd 2008 and from October 16th 2008 to April 15th 2009). AA thanks CSIC for the Ph.D. contract 5072160068 W0SC000077 within the A1 group. This work has been partially defrayed by the grant AGL2010-21806-C02-01 (Spanish Ministry of Science, MICINN), RICET (RETICS-FIS, FEDER) collaborative network grant and the Fundación Ramón Areces contract 050204100014/OTT code 20100338. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.es_ES
dc.language.isoenges_ES
dc.publisherPublic Library of Science (PLOS) es_ES
dc.type.hasVersionVoRes_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.meshAgglutination es_ES
dc.subject.meshCrithidia fasciculata es_ES
dc.subject.meshHomeostasis es_ES
dc.subject.meshKinetics es_ES
dc.subject.meshLeishmania es_ES
dc.subject.meshLipid Metabolism es_ES
dc.subject.meshOxidation-Reduction es_ES
dc.subject.meshPeanut Agglutinin es_ES
dc.subject.meshPentose Phosphate Pathway es_ES
dc.subject.meshProtozoan Proteins es_ES
dc.subject.meshSignal Transduction es_ES
dc.subject.meshSpecies Specificity es_ES
dc.subject.meshSulfhydryl Compounds es_ES
dc.subject.meshTranscriptome es_ES
dc.subject.meshAxenic Culture es_ES
dc.subject.meshProteomics es_ES
dc.titleAn Insight into the proteome of Crithidia fasciculata choanomastigotes as a comparative approach to axenic growth, peanut lectin agglutination and differentiation of Leishmania spp. promastigoteses_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID25503511es_ES
dc.format.volume9es_ES
dc.format.number12es_ES
dc.format.pagee113837es_ES
dc.identifier.doi10.1371/journal.pone.0113837es_ES
dc.contributor.funderMinisterio de Ciencia e Innovación (España) 
dc.contributor.funderUnión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) 
dc.contributor.funderFundación Ramón Areces 
dc.description.peerreviewedes_ES
dc.identifier.e-issn1932-6203es_ES
dc.relation.publisherversionhttps://doi.org/10.1371/journal.pone.0113837es_ES
dc.identifier.journalPloS onees_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/AGL2010-21806-C02-01es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/050204100014 / código OTT 20100338es_ES
dc.rights.accessRightsopen accesses_ES


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Atribución 4.0 Internacional
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