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dc.contributor.authorBotana, Laura 
dc.contributor.authorIbarra-Meneses, Ana Victoria 
dc.contributor.authorSanchez Herrero, Carmen 
dc.contributor.authorCastro, Alicia
dc.contributor.authorSan Martín, Juan Víctor
dc.contributor.authorMolina, Laura
dc.contributor.authorRuiz-Giardin, José Manuel
dc.contributor.authorCarrillo, Eugenia 
dc.contributor.authorMoreno, Javier 
dc.date.accessioned2019-11-25T12:56:41Z
dc.date.available2019-11-25T12:56:41Z
dc.date.issued2019
dc.identifier.citationPLoS Negl Trop Dis. 2019 Jun 3;13(6):e0007461.es_ES
dc.identifier.issn1935-2735es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/8699
dc.description.abstractConcomitant infection with human immunodeficiency virus (HIV) and the Leishmania parasite is a growing public health problem, the result of the former spreading to areas where the latter is endemic. Leishmania infection is usually asymptomatic in immunocompetent individuals, but the proportion of HIV+ individuals in contact with the parasite who remain asymptomatic is not known. The aim of the present work was to examine the use of cytokine release assays in the detection of asymptomatic immune responders to Leishmania among HIV+ patients with no previous leishmaniasis or current symptomatology. Eighty two HIV+ patients (all from Fuenlabrada, Madrid, Spain, where a leishmaniasis outbreak occurred in 2009) were examined for Leishmania infantum infection using molecular and humoral response-based methods. None returned a positive molecular or serological result for the parasite. Thirteen subjects showed a positive lymphoproliferative response to soluble Leishmania antigen (SLA), although the mean CD4+ T lymphocyte counts of these patients was below the normal range. Stimulation of peripheral blood mononuclear cells (PBMC) or whole blood with SLA (the lymphoproliferative assay and whole blood assay respectively), led to the production of specific cytokines and chemokines. Thus, despite being immunocompromised, HIV+ patients can maintain a Th1-type cellular response to Leishmania. In addition, cytokine release assays would appear to be useful tools for detecting these individuals via the identification of IFN-γ in the supernatants of SLA-stimulated PBMC, and of IFN-γ, MIG and IL-2 in SLA-stimulated whole blood. These biomarkers appear to be 100% reliable for detecting asymptomatic immune responders to Leishmania among HIV+ patients.es_ES
dc.description.sponsorshipThis work was funded by the Instituto de Salud Carlos III via the Red de Enfermedades Tropicales, Subprograma RETICS del Plan Estatal de I+D+I 2013-2016, which is co-funded by FEDER “Una manera de hacer Europa” funds, via projects RD16/0027/0017 and RD16CIII/0003/0002. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.es_ES
dc.language.isoenges_ES
dc.publisherPublic Library of Sciencees_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleAsymptomatic immune responders to Leishmania among HIV positive patientses_ES
dc.typeArtículoes_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID31158223es_ES
dc.format.volume13es_ES
dc.format.number6es_ES
dc.format.pagee0007461es_ES
dc.identifier.doi10.1371/journal.pntd.0007461es_ES
dc.contributor.funderInstituto de Salud Carlos III - ISCIIIes_ES
dc.contributor.funderRed de Investigación Cooperativa en Enfermedades Tropicales (España)es_ES
dc.contributor.funderEuropean Regional Development Fund (ERDF/FEDER)es_ES
dc.description.peerreviewedes_ES
dc.identifier.e-issn1935-2735es_ES
dc.relation.publisherversionhttps://doi.org/10.1371/journal.pntd.0007461es_ES
dc.identifier.journalPLoS neglected tropical diseaseses_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/RD16/0027/0017es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/RD16CIII/0003/0002es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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Atribución 4.0 Internacional
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