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dc.contributor.authorGonzalez, Luis Miguel 
dc.contributor.authorEstrada, Karel
dc.contributor.authorGrande, Ricardo
dc.contributor.authorJiménez-Jacinto, Verónica
dc.contributor.authorVega-Alvarado, Leticia
dc.contributor.authorSevilla, Elena 
dc.contributor.authorBarrera, Jorge de la 
dc.contributor.authorCuesta, Isabel 
dc.contributor.authorZaballos, Angel 
dc.contributor.authorBautista, José Manuel
dc.contributor.authorLobo, Cheryl A
dc.contributor.authorSánchez-Flores, Alejandro
dc.contributor.authorMontero-Clemente, Estrella
dc.identifier.citationPLoS Negl Trop Dis. 2019 Aug 19;13(8):e0007680.es_ES
dc.description.abstractBabesiosis is considered an emerging disease because its incidence has significantly increased in the last 30 years, providing evidence of the expanding range of this rare but potentially life-threatening zoonotic disease. Babesia divergens is a causative agent of babesiosis in humans and cattle in Europe. The recently sequenced genome of B. divergens revealed over 3,741 protein coding-genes and the 10.7-Mb high-quality draft become the first reference tool to study the genome structure of B. divergens. Now, by exploiting this sequence data and using new computational tools and assembly strategies, we have significantly improved the quality of the B. divergens genome. The new assembly shows better continuity and has a higher correspondence to B. bovis chromosomes. Moreover, we present a differential expression analysis using RNA sequencing of the two different stages of the asexual lifecycle of B. divergens: the free merozoite capable of invading erythrocytes and the intraerythrocytic parasite stage that remains within the erythrocyte until egress. Comparison of mRNA levels of both stages identified 1,441 differentially expressed genes. From these, around half were upregulated and the other half downregulated in the intraerythrocytic stage. Orthogonal validation by real-time quantitative reverse transcription PCR confirmed the differential expression. A moderately increased expression level of genes, putatively involved in the invasion and egress processes, were revealed in the intraerythrocytic stage compared with the free merozoite. On the basis of these results and in the absence of molecular models of invasion and egress for B. divergens, we have proposed the identified genes as putative molecular players in the invasion and egress processes. Our results contribute to an understanding of key parasitic strategies and pathogenesis and could be a valuable genomic resource to exploit for the design of diagnostic methods, drugs and vaccines to improve the control of babesiosis.es_ES
dc.description.sponsorshipThis work was funded by grants from Ministerio de Economía y Competitividad from Spain (AGL2010-21774 and AGL2014-56193 R to EM and LMG). ES was awarded a research fellowship from Plan Estatal de Investigación Científica y Técnica y de Innovación, Ministerio de Economía y Competitividad, Spain ( Work in CL’s laboratory is funded by a grant from the National Institutes of Health ( NIH- 1R01HL140625-01. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscriptes_ES
dc.publisherPublic Library of Sciencees_ES
dc.relation.isversionofPublisher's versiones_ES
dc.titleComparative and functional genomics of the protozoan parasite Babesia divergens highlighting the invasion and egress processeses_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.contributor.funderMinisterio de Economía y Competitividad (España)
dc.identifier.journalPLoS neglected tropical diseaseses_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES

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