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dc.contributor.authorCortegano, Isabel 
dc.contributor.authorSerrano, Natalia
dc.contributor.authorRuiz, Carolina 
dc.contributor.authorDominguez-Rodriguez, Mercedes 
dc.contributor.authorPrado-Zamora, Maria Carmen 
dc.contributor.authorAlia, Mario 
dc.contributor.authorHidalgo, Andres 
dc.contributor.authorCano, Eva 
dc.contributor.authorAndres, Belen de 
dc.contributor.authorGaspar, Maria Luisa 
dc.date.accessioned2019-10-17T08:14:41Z
dc.date.available2019-10-17T08:14:41Z
dc.date.issued2019-09
dc.identifier.citationHaematologica. 2019, 104(9): 1853-65es_ES
dc.identifier.issn0390-6078es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/8502
dc.description.abstractEmbryonic megakaryopoiesis starts in the yolk sac on gestational day 7.5 as part of the primitive wave of hematopoiesis, and it continues in the fetal liver when this organ is colonized by hematopoietic progenitors between day 9.5 and 10.5, as the definitive hematopoiesis wave. We characterized the precise phenotype of embryo megakaryocytes in the liver at gestational day 11.5, identifying them as CD41++CD45-CD9++CD61+MPL+CD42c+ tetraploid cells that express megakaryocyte-specific transcripts and display differential traits when compared to those present in the yolk sac at the same age. In contrast to megakaryocytes from adult bone marrow, embryo megakaryocytes are CD45- until day 13.5 of gestation, as are both the megakaryocyte progenitors and megakaryocyte/erythroid-committed progenitors. At gestational day 11.5, liver and yolk sac also contain CD41+CD45+ and CD41+CD45- cells. These populations, and that of CD41++CD45-CD42c+ cells, isolated from liver, differentiate in culture into CD41++CD45-CD42c+ proplatelet-bearing megakaryocytes. Also present at this time are CD41-CD45++CD11b+ cells, which produce low numbers of CD41++CD45-CD42c+ megakaryocytes in vitro, as do fetal liver cells expressing the macrophage-specific Csf receptor-1 (Csf1r/CD115) from MaFIA transgenic mice, which give rise poorly to CD41++CD45-CD42c+ embryo megakaryocytes both in vivo and in vitro In contrast, around 30% of adult megakaryocytes (CD41++CD45++CD9++CD42c+) from C57BL/6 and MaFIA mice express CD115. We propose that differential pathways operating in the mouse embryo liver at gestational day 11.5 beget CD41++CD45-CD42c+ embryo megakaryocytes that can be produced from CD41+CD45- or from CD41+CD45+ cells, at difference from those from bone marrow.es_ES
dc.description.sponsorshipThis work was supported by grants from the Ministerio de Ciencia e Innovacion (MICINN SAF2009-12596) and from the Ministerio de Economia y Competitividad (MINECO SAF2012-33916 and SAF2015-70880-R MINECO/FEDER). NS was the recipient of a fellowship from the Centro de Biologia Molecular Severo Ochoa (CBMSO) and IC received a fellowship from the MICINN. The CBMSO receives institutional funding from Fundacion Ramon Areces. The CNIC is supported by the MEIC and the Pro CNIC Foundation, and is a Severo Ochoa Center of Excellence (MEIC award SEV-2015-0505).es_ES
dc.language.isoenges_ES
dc.type.hasVersionVoRes_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/*
dc.titleCD45 expression discriminates waves of embryonic megakaryocytes in the mousees_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución-NoComercial 4.0 Internacional*
dc.identifier.pubmedID30573502es_ES
dc.format.volume104es_ES
dc.format.number9es_ES
dc.format.page1853-1865es_ES
dc.identifier.doi10.3324/haematol.2018.192559es_ES
dc.contributor.funderMinisterio de Ciencia e Innovación (España) 
dc.contributor.funderMinisterio de Economía y Competitividad (España) 
dc.contributor.funderFundación Ramón Areces 
dc.contributor.funderFundación ProCNIC 
dc.contributor.funderUnión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) 
dc.description.peerreviewedes_ES
dc.identifier.e-issn1592-8721es_ES
dc.identifier.journalHaematologicaes_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Imagen de la Inflamación Cardiovascular y la Respuesta Inmunees_ES
dc.repisalud.institucionCNICes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SEV-2015-0505es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2009-12596es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2012-33916es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2015-70880-Res_ES
dc.rights.accessRightsopen accesses_ES


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