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dc.contributor.authorMoll, Herwig P
dc.contributor.authorMohrherr, Julian
dc.contributor.authorBlaas, Leander
dc.contributor.authorMusteanu, Mónica 
dc.contributor.authorStiedl, Patricia
dc.contributor.authorGrabner, Beatrice
dc.contributor.authorZboray, Katalin
dc.contributor.authorKönig, Margit
dc.contributor.authorStoiber, Dagmar
dc.contributor.authorRülicke, Thomas
dc.contributor.authorStrehl, Sabine
dc.contributor.authorEferl, Robert
dc.contributor.authorCasanova, Emilio
dc.identifier.citationCancers (Basel). 2019;11(9). pii: E1226.es_ES
dc.description.abstractGenetically-engineered mouse models (GEMMs) lacking diseased-associated gene(s) globally or in a tissue-specific manner represent an attractive tool with which to assess the efficacy and toxicity of targeted pharmacological inhibitors. Stat3 and Stat5a/b transcription factors have been implicated in several pathophysiological conditions, and pharmacological inhibition of both transcription factors has been proposed to treat certain diseases, such as malignancies. To model combined inhibition of Stat3 and Stat5a/b we have developed a GEMM harboring a flox Stat3-Stat5a/b allele (Stat5/3loxP/loxP mice) and generated mice lacking Stat3 and Stat5a/b in hepatocytes (Stat5/3Δhep/Δhep). Stat5/3Δhep/Δhep mice exhibited a marked reduction of STAT3, STAT5A and STAT5B proteins in the liver and developed steatosis, a phenotype that resembles mice lacking Stat5a/b in hepatocytes. In addition, embryonic deletion of Stat3 and Stat5a/b (Stat5/3Δ/Δ mice) resulted in lethality, similar to Stat3Δ/Δ mice. This data illustrates that Stat5/3loxP/loxP mice are functional and can be used as a valuable tool to model the combined inhibition of Stat3 and Stat5a/b in tumorigenesis and other diseases.es_ES
dc.description.sponsorshipWe thank Hans-Christian Theussl for performing blastocyst injections, Francis Stewart for providing the pUBC/EM7-hygromicin and pSC101-BAD-gbaA plasmids.Funding: Open Access Funding by the Austrian Science Fund (FWF): P 25599-B19 (EC) and the Austrian Federal Ministry of Science and Research GENAU grant ‘Austromouse’ (EC and RE). RE was supported by the Austrian Science Fund (FWF) Doktoratskolleg-plus grant “Inflammation and Immunity”, the FWF grants P25925-B20,P26908-B20 and P29222-B28.es_ES
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI) es_ES
dc.subjectbacterial artificial chromosomees_ES
dc.subjectembryonic stem cellses_ES
dc.subjectgene targetinges_ES
dc.subjectliver steatosises_ES
dc.titleA Mouse Model to Assess STAT3 and STAT5A/B Combined Inhibition in Health and Disease Conditionses_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución-NoComercial-CompartirIgual 4.0 Internacional*
dc.contributor.funderAustrian Science Fund
dc.contributor.funderAustrian Federal Ministry of Science and Research
dc.repisalud.orgCNIOCNIO::Grupos de investigación::Grupo de Oncología Experimentales_ES
dc.rights.accessRightsopen accesses_ES

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Atribución-NoComercial-CompartirIgual 4.0 Internacional
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