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dc.contributor.author | Ferrandiz-Avellano, Maria-Jose | |
dc.contributor.author | Ardanuy, Carmen | |
dc.contributor.author | Liñares, Josefina | |
dc.contributor.author | García-Arenzana, José María | |
dc.contributor.author | Cercenado, Emilia | |
dc.contributor.author | Fleites, Ana | |
dc.contributor.author | de la Campa, Adela G | |
dc.date.accessioned | 2019-08-14T07:25:39Z | |
dc.date.available | 2019-08-14T07:25:39Z | |
dc.date.issued | 2005-06 | |
dc.identifier.citation | Antimicrob Agents Chemother. 2005 Jun;49(6):2237-45. | es_ES |
dc.identifier.issn | 0066-4804 | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/8271 | |
dc.description.abstract | A total of 103 (0.7%) of 14,236 Streptococcus pneumoniae isolates collected in four Spanish hospitals from 1989 to 2003 were resistant to rifampin (MICs, 4 to 512 microg/ml). Only sixty-one (59.2%) of these isolates were available for molecular characterization. Resistance was mostly related to human immunodeficiency virus (HIV) infection in adult patients and to conjunctivitis in children. Thirty-six different pulsed-field gel electrophoresis patterns were identified among resistant isolates, five of which were related to international clones (Spain23F-1, Spain6B-2, Spain9V-3, Spain14-5, and clone C of serotype 19F), and accounted for 49.2% of resistant isolates. Single sense mutations at cluster N or I of the rpoB gene were found in 39 isolates, while double mutations, either at cluster I, at clusters I and II, or at clusters N and III, were found in 14 isolates. The involvement of the mutations in rifampin resistance was confirmed by genetic transformation. Single mutations at clusters N and I conferred MICs of 2 microg/ml and 4 to 32 microg/ml, respectively. Eight isolates showed high degrees of nucleotide sequence variations (2.3 to 10.8%) in rpoB, suggesting a recombinational origin for these isolates, for which viridans group streptococci are their potential gene donors. Although the majority of rifampin-resistant isolates were isolated from individual patients without temporal or geographical relationships, the clonal dissemination of rifampin-resistant isolates was observed among 12 HIV-infected patients in the two hospitals with higher rates of resistance. | es_ES |
dc.description.sponsorship | This work was supported by Red Temática de Investigación Cooperativa G03/103 from Fondo de Investigación Sanitaria and by grant BIO2002-01398 from the Ministerio de Ciencia y Tecnología. We acknowledge the use of the pneumococcal MLST database, which is located at Imperial College London and which is funded by the Wellcome Trust | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | American Society for Microbiology (ASM) | es_ES |
dc.type.hasVersion | AM | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | * |
dc.subject.mesh | Amino Acid Sequence | es_ES |
dc.subject.mesh | Anti-Bacterial Agents | es_ES |
dc.subject.mesh | Base Sequence | es_ES |
dc.subject.mesh | DNA-Directed RNA Polymerases | es_ES |
dc.subject.mesh | Drug Resistance, Bacterial | es_ES |
dc.subject.mesh | Hospitals | es_ES |
dc.subject.mesh | Humans | es_ES |
dc.subject.mesh | Microbial Sensitivity Tests | es_ES |
dc.subject.mesh | Molecular Sequence Data | es_ES |
dc.subject.mesh | Phylogeny | es_ES |
dc.subject.mesh | Pneumococcal Infections | es_ES |
dc.subject.mesh | Rifampin | es_ES |
dc.subject.mesh | Sequence Analysis, DNA | es_ES |
dc.subject.mesh | Spain | es_ES |
dc.subject.mesh | Streptococcus pneumoniae | es_ES |
dc.subject.mesh | Gene Transfer, Horizontal | es_ES |
dc.subject.mesh | Mutation | es_ES |
dc.title | New mutations and horizontal transfer of rpoB among rifampin-resistant Streptococcus pneumoniae from four Spanish hospitals | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Atribución-NoComercial-CompartirIgual 4.0 Internacional | * |
dc.identifier.pubmedID | 15917517 | es_ES |
dc.format.volume | 49 | es_ES |
dc.format.number | 6 | es_ES |
dc.format.page | 2237-45 | es_ES |
dc.identifier.doi | 10.1128/AAC.49.6.2237-2245.2005 | es_ES |
dc.contributor.funder | Instituto de Salud Carlos III | |
dc.relation.publisherversion | https://doi.org/10.1128/AAC.49.6.2237-2245.2005 | es_ES |
dc.identifier.journal | Antimicrobial agents and chemotherapy | es_ES |
dc.repisalud.centro | ISCIII::Centro Nacional de Microbiología | es_ES |
dc.repisalud.institucion | ISCIII | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/BIO2002-01398 | es_ES |
dc.rights.accessRights | open access | es_ES |