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dc.contributor.authorMartin-Galiano, Antonio Javier 
dc.contributor.authorBalsalobre-Arenas, Maria Luz 
dc.contributor.authorFenoll, Asuncion 
dc.contributor.authorde la Campa, Adela G 
dc.date.accessioned2019-08-13T12:18:59Z
dc.date.available2019-08-13T12:18:59Z
dc.date.issued2003-10
dc.identifier.citationAntimicrob Agents Chemother. 2003 Oct;47(10):3187-94.es_ES
dc.identifier.issn0066-4804es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/8244
dc.description.abstractTwo clinical isolates of viridans group streptococci (VS) with different degrees of susceptibility to optochin (OPT), i.e., fully OPT-susceptible (Opt(s)) VS strain 1162/99 (for which the MIC was equal to that for Streptococcus pneumoniae, 0.75 micro g/ml) and intermediate Opt(s) VS strain 1174/97 (MIC, 6 micro g/ml) were studied. Besides being OPT susceptible, they showed characteristics typical of VS, such as bile insolubility; lack of reaction with pneumococcal capsular antibodies; and lack of hybridization with rRNA (AccuProbe)-, lytA-, and pnl-specific pneumococcal probes. However, these VS Opt(s) strains and VS type strains hybridized with ant, a gene not present in S. pneumoniae. A detailed characterization of the genes encoding the 16S rRNA and SodA classified isolates 1162/99 and 1174/97 as Streptococcus mitis. Analysis of the atpCAB region, which encodes the c, a, and b subunits of the F(0)F(1) H(+)-ATPase, the target of optochin, revealed high degrees of similarity between S. mitis 1162/99 and S. pneumoniae in atpC, atpA, and the N terminus of atpB. Moreover, amino acid identity between S. mitis 1174/97 and S. pneumoniae was found in alpha helix 5 of the a subunit. The organization of the chromosomal region containing the atp operon of the two Opt(s) VS and VS type strains was spr1284-atpC, with spr1284 being located 296 to 556 bp from atpC, whereas in S. pneumoniae this distance was longer than 68 kb. In addition, the gene order in S. pneumoniae was IS1239-74 bp-atpC. The results suggest that the full OPT susceptibility of S. mitis 1162/99 is due to the acquisition of atpC, atpA, and part of atpB from S. pneumoniae and that the intermediate OPT susceptibility of S. mitis 1174/97 correlates with the amino acid composition of its a subunit.es_ES
dc.description.sponsorshipWe thank E. García for critical reading of the manuscript. The technical assistance of G. Asensio and A. Rodríguez-Bernabé is acknowledged. A.J.M.-G. and L.B. received fellowships from the Comunidad Autónoma de Madrid and the Instituto de Salud Carlos III, respectively. This study was supported by grant 1274/01 from the Instituto de Salud Carlos III and grant BIO2002-01398 from the Ministerio de Ciencia y Tecnología.es_ES
dc.language.isoenges_ES
dc.publisherAmerican Society for Microbiologyes_ES
dc.relation.isversionofPreprintes_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subject.meshAmino Acid Sequence es_ES
dc.subject.meshAmino Alcohols es_ES
dc.subject.meshAntimalarials es_ES
dc.subject.meshBase Sequence es_ES
dc.subject.meshChromosome Mapping es_ES
dc.subject.meshGenes, Bacterial es_ES
dc.subject.meshMicrobial Sensitivity Tests es_ES
dc.subject.meshMolecular Sequence Data es_ES
dc.subject.meshNucleic Acid Hybridization es_ES
dc.subject.meshPhylogeny es_ES
dc.subject.meshPolymorphism, Genetices_ES
dc.subject.meshQuinine es_ES
dc.subject.meshRecombination, Genetices_ES
dc.subject.meshViridans Streptococci es_ES
dc.titleGenetic characterization of optochin-susceptible viridans group streptococcies_ES
dc.typeArtículoes_ES
dc.rights.licenseAtribución-NoComercial-CompartirIgual 4.0 Internacional*
dc.identifier.pubmedID14506029es_ES
dc.format.volume47es_ES
dc.format.number10es_ES
dc.format.page3187-94es_ES
dc.identifier.doi10.1128/aac.47.10.3187-3194.2003es_ES
dc.contributor.funderInstituto de Salud Carlos III - ISCIIIes_ES
dc.contributor.funderComunidad de Madrides_ES
dc.contributor.funderMinisterio de Ciencia y Tecnología (España)es_ES
dc.relation.publisherversionhttps://doi.org/10.1128/aac.47.10.3187-3194.2003es_ES
dc.identifier.journalAntimicrobial agents and chemotherapyes_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/1274/01es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/BIO2002-01398es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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