Publication: A point mutation in the 14alpha-sterol demethylase gene cyp51A contributes to itraconazole resistance in Aspergillus fumigatus
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Identifiers
Publication date
2003-03
Authors
Diaz-Guerra, Teresa M. ISCIII
Mellado, Emilia ISCIII
Cuenca-Estrella, Manuel ISCIII
Rodriguez-Tudela, Juan Luis ISCIII
Advisors
Journal Title
Journal ISSN
Volume Title
Publisher
American Society for Microbiology (ASM)
Abstract
The genes encoding 14alpha-sterol demethylases (cyp51A and cyp51B) were analyzed in 12 itraconazole (ITC)-resistant and three ITC-susceptible clinical isolates of Aspergillus fumigatus. Six ITC-resistant strains exhibited a substitution of another amino acid for glycine at position 54, which is located at a very conserved region of the Cyp51A protein. The cyp51A gene from the A. fumigatus wild-type strain (CM-237) was replaced with the mutated cyp51A gene copy of an ITC-resistant strain (AF-72). Two transformants exhibited resistance to ITC, both of which had incorporated the mutated copy of the cyp51A gene.
Description
Erratum in: Antimicrob Agents Chemother. 2004 Mar;48(3):1071.
Keywords
MeSH Terms
Amino Acid Substitution Antifungal Agents Aspergillus fumigatus Base Sequence Cytochrome P-450 Enzyme System Drug Resistance, Fungal Electroporation Fungal Proteins Itraconazole Microbial Sensitivity Tests Molecular Sequence Data Point Mutation Reverse Transcriptase Polymerase Chain Reaction Transformation, Genetic
DeCS Terms
Bibliographic citation
Antimicrob Agents Chemother. 2003 Mar;47(3):1120-4.
Publisher version
Document type
journal article