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dc.contributor.authorGonzalez Portal, Maria Eugenia 
dc.contributor.authorCarrasco, Luis
dc.identifier.citationBiochemistry 37 (39) 13710-13719es_ES
dc.description.abstractInfection of T lymphocytes by the human immunodeficiency virus causes drastic alterations in the intracellular cation content of the infected cells. The human immunodeficiency virus type 1 genome encodes several accessory proteins, including Vpu, an integral membrane protein that forms ion channels in planar lipid bilayers. The effect of Vpu on the permeability of the plasma membrane to several molecules has been analyzed. Expression of vpu in Escherichia coli cells increases membrane permeability to a number of molecules such as 2-nitrophenyl beta-D-galactopyranoside, uridine, the impermeable translation inhibitor hygromycin B, and lysozyme. In addition, transient expression of Vpu in eukaryotic COS cells enhances entry of charged molecules such as hygromycin B and neurobiotin into these cells. The effect of Vpu on cell membrane permeability resembles that reported for other membrane-active proteins from different animal viruses, including influenza M2, Semliki Forest virus 6K, and poliovirus 2B and 3A proteins.es_ES
dc.publisherAmerican Chemical Society (ACS) es_ES
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dc.subjectHIV-1 Vpu proteines_ES
dc.subjectMembrane permeabilizationes_ES
dc.subjectRecombinant expressiones_ES
dc.subject.meshAnimals es_ES
dc.subject.meshCOS Cells es_ES
dc.subject.meshCercopithecus aethiopses_ES
dc.subject.meshCloning, Moleculares_ES
dc.subject.meshEscherichia coli es_ES
dc.subject.meshGene Expression Regulation, Viral es_ES
dc.subject.meshHIV-1 es_ES
dc.subject.meshHuman Immunodeficiency Virus Proteins es_ES
dc.subject.meshHumans es_ES
dc.subject.meshSubcellular Fractions es_ES
dc.subject.meshTransfection es_ES
dc.subject.meshViral Regulatory and Accessory Proteins es_ES
dc.subject.meshCell Membrane Permeability es_ES
dc.titleThe Human Immunodeficiency Virus Type 1 Vpu Protein Enhances Membrane Permeability†es_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución-NoComercial-CompartirIgual 4.0 Internacional*
dc.contributor.funderInstituto de Salud Carlos III 
dc.contributor.funderBristol-Myers Squibb 
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.rights.accessRightsopen accesses_ES

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