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dc.contributor.authorChevre, Raphael 
dc.contributor.authorGonzalez-Granado, Jose Maria 
dc.contributor.authorMegens, Remco T A
dc.contributor.authorSreeramkumar, Vinatha 
dc.contributor.authorSilvestre-Roig, Carlos 
dc.contributor.authorMolina-Sanchez, Pedro 
dc.contributor.authorWeber, Christian
dc.contributor.authorSoehnlein, Oliver
dc.contributor.authorHidalgo, Andrés 
dc.contributor.authorAndres, Vicente 
dc.date.accessioned2019-07-17T07:10:58Z
dc.date.available2019-07-17T07:10:58Z
dc.date.issued2014-02
dc.identifier.citationCirc Res. 2014; 114(5):770-9es_ES
dc.identifier.issn0009-7330es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/7918
dc.description.abstractRATIONALE: The inflammatory processes that initiate and propagate atherosclerosis remain poorly understood, largely because defining the intravascular behavior of immune cells has been technically challenging. Respiratory and pulsatile movements have hampered in vivo visualization of leukocyte accumulation in athero-prone arteries at resolutions achieved in other tissues. OBJECTIVE: To establish and to validate a method that allows high-resolution imaging of inflammatory leukocytes and platelets within the carotid artery of atherosusceptible mice in vivo. METHODS AND RESULTS: We have devised a procedure to stabilize the mouse carotid artery mechanically without altering blood dynamics, which dramatically enhances temporal and spatial resolutions using high-speed intravital microscopy in multiple channels of fluorescence. By applying this methodology at different stages of disease progression in atherosusceptible mice, we first validated our approach by assessing the recruitment kinetics of various leukocyte subsets and platelets in athero-prone segments of the carotid artery. The high temporal and spatial resolution allowed the dissection of both the dynamic polarization of and the formation of subcellular domains within adhered leukocytes. We further demonstrate that the secondary capture of activated platelets on the plaque is predominantly mediated by neutrophils. Finally, we couple this procedure with triggered 2-photon microscopy to visualize the 3-dimensional movement of leukocytes in intimate contact with the arterial lumen. CONCLUSIONS: The improved imaging of diseased arteries at subcellular resolution presented here should help resolve many outstanding questions in atherosclerosis and other arterial disorders.es_ES
dc.description.sponsorshipSpanish Ministry of Economy and Competitivity (MINECO) [SAF2009-11037, SAF2010-16044]; European Commission [FP7-People-IRG 246655, Liphos-317916]; Instituto de Salud Carlos III (ISCIII) [RD12/0042/0028]; Ramon y Cajal fellowship [(RYC-2007-00697]; ISCIII [CP11/00145]; Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NWO) (VIDI project) [91712303]; Deutsche Forschungsgemeinschaft (DFG) [SO876/3-1, SO876/6-1, FOR809, SFB914-B08, WE1913/11-2, KI 1072/8-1, INST409/97-1]; Else Kroner Fresenius Stiftung; LMUexcellence initiative; European Research Council [ERC AdGo 249929]; Friedrich Baur Stiftung; MINECO; Pro-CNIC Foundationes_ES
dc.language.isoenges_ES
dc.publisherAmerican Heart Associationes_ES
dc.relation.isversionofPostprintes_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectatherosclerosises_ES
dc.subjectblood plateletses_ES
dc.subjectcarotid arterieses_ES
dc.subjectneutrophilses_ES
dc.subject.meshAnimals es_ES
dc.subject.meshApolipoproteins E es_ES
dc.subject.meshAtherosclerosis es_ES
dc.subject.meshBlood Platelets es_ES
dc.subject.meshCarotid Artery Diseases es_ES
dc.subject.meshCarotid Artery, Common es_ES
dc.subject.meshFemale es_ES
dc.subject.meshGreen Fluorescent Proteins es_ES
dc.subject.meshLeukocyte Rolling es_ES
dc.subject.meshLeukocytes es_ES
dc.subject.meshMale es_ES
dc.subject.meshMice es_ES
dc.subject.meshMice, Knockout es_ES
dc.subject.meshMicroscopy, Fluorescence es_ES
dc.subject.meshMyeloid Cells es_ES
dc.subject.meshNeutrophils es_ES
dc.subject.meshRegional Blood Flow es_ES
dc.subject.meshVasculitis es_ES
dc.titleHigh-resolution imaging of intravascular atherogenic inflammation in live micees_ES
dc.typeArtículoes_ES
dc.rights.licenseAtribución-NoComercial-CompartirIgual 4.0 Internacional*
dc.identifier.pubmedID24366169es_ES
dc.format.volume114es_ES
dc.format.number5es_ES
dc.format.page770-9es_ES
dc.identifier.doi10.1161/CIRCRESAHA.114.302590es_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.contributor.funderEuropean Commissiones_ES
dc.contributor.funderInstituto de Salud Carlos III - ISCIIIes_ES
dc.contributor.funderNederlandse Organisatie voor Wetenschappelijk Onderzoekes_ES
dc.contributor.funderDeutsche Forschungsgemeinschaftes_ES
dc.contributor.funderEuropean Research Counciles_ES
dc.contributor.funderFriedrich Baur Stiftunges_ES
dc.contributor.funderFundación ProCNICes_ES
dc.description.peerreviewedes_ES
dc.identifier.e-issn1524-4571es_ES
dc.relation.publisherversionhttps://doi.org/10.1161/CIRCRESAHA.114.302590es_ES
dc.identifier.journalCirculation researches_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Fisiopatología Cardiovascular Molecular y Genéticaes_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Imagen de la Inflamación Cardiovascular y la Respuesta Inmunees_ES
dc.repisalud.institucionCNICes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/249929/EUes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2009-11037es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2010-16044es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/246655/EUes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/317916/EUes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/RD12/0042/0028es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/RYC-2007-00697es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/CP11/00145es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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Atribución-NoComercial-CompartirIgual 4.0 Internacional
This item is licensed under a: Atribución-NoComercial-CompartirIgual 4.0 Internacional