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dc.contributor.authorAssi, Hala Abou
dc.contributor.authorHarkness, Robert W
dc.contributor.authorMartin-Pintado, Nerea
dc.contributor.authorWilds, Christopher J
dc.contributor.authorCampos Olivas, Ramon 
dc.contributor.authorMittermaier, Anthony K
dc.contributor.authorGonzalez, Carlos 
dc.contributor.authorDamha, Masad J
dc.identifier.citationNucleic Acids Res. 2016;44(11):4998-5009.es_ES
dc.description.abstracti-Motifs are four-stranded DNA structures consisting of two parallel DNA duplexes held together by hemi-protonated and intercalated cytosine base pairs (C:CH(+)). They have attracted considerable research interest for their potential role in gene regulation and their use as pH responsive switches and building blocks in macromolecular assemblies. At neutral and basic pH values, the cytosine bases deprotonate and the structure unfolds into single strands. To avoid this limitation and expand the range of environmental conditions supporting i-motif folding, we replaced the sugar in DNA by 2-deoxy-2-fluoroarabinose. We demonstrate that such a modification significantly stabilizes i-motif formation over a wide pH range, including pH 7. Nuclear magnetic resonance experiments reveal that 2-deoxy-2-fluoroarabinose adopts a C2'-endo conformation, instead of the C3'-endo conformation usually found in unmodified i-motifs. Nevertheless, this substitution does not alter the overall i-motif structure. This conformational change, together with the changes in charge distribution in the sugar caused by the electronegative fluorine atoms, leads to a number of favorable sequential and inter-strand electrostatic interactions. The availability of folded i-motifs at neutral pH will aid investigations into the biological function of i-motifs in vitro, and will expand i-motif applications in nanotechnology.es_ES
dc.description.sponsorshipThis work is dedicated to the Memory of Alfredo Villasante, valuable collaborator and friend. FUNDING Funding for open access charge: NSERC Discovery grant (to M.J.D., A.K.M.); CIHR DDTP Training Grant (to H.A., R.H.V.); MINECO [BFU2014-52864-R to C.G.]; CSIC-JAE contract (to N.M.P.). Conflict of interest statement. None declaredes_ES
dc.publisherOxford University Press es_ES
dc.subject.meshCytosine es_ES
dc.subject.meshDNA es_ES
dc.subject.meshHydrogen-Ion Concentration es_ES
dc.subject.meshIntercalating Agents es_ES
dc.subject.meshMagnetic Resonance Spectroscopy es_ES
dc.subject.meshThermodynamics es_ES
dc.subject.meshBase Pairing es_ES
dc.subject.meshNucleic Acid Conformation es_ES
dc.subject.meshNucleotide Motifs es_ES
dc.titleStabilization of i-motif structures by 2'-β-fluorination of DNAes_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución-NoComercial-CompartirIgual 4.0 Internacional*
dc.contributor.funderMinisterio de Economía y Competitividad (España)
dc.identifier.journalNucleic acids researches_ES
dc.repisalud.orgCNIOCNIO::Unidades técnicas::Unidad de Espectroscopía y RMNes_ES
dc.rights.accessRightsopen accesses_ES

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Atribución-NoComercial-CompartirIgual 4.0 Internacional
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