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dc.contributor.authorRichart, Laia
dc.contributor.authorCarrillo-de Santa Pau, Enrique
dc.contributor.authorRío-Machín, Ana
dc.contributor.authorde Andrés, Mónica P
dc.contributor.authorCigudosa, Juan C
dc.contributor.authorLobo, Víctor J Sánchez-Arévalo
dc.contributor.authorReal Arribas, Francisco 
dc.date.accessioned2019-07-04T11:32:40Z
dc.date.available2019-07-04T11:32:40Z
dc.date.issued2016-01-05
dc.identifier.citationNat Commun. 2016 ;7:10153.es_ES
dc.identifier.issn2041-1723es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/7856
dc.description.abstractc-MYC oncogene is deregulated in most human tumours. Histone marks associated with transcriptionally active genes define high-affinity c-MYC targets. The mechanisms involved in their recognition by c-MYC are unknown. Here we report that c-MYC interacts with BPTF, a core subunit of the NURF chromatin-remodelling complex. BPTF is required for the activation of the full c-MYC transcriptional programme in fibroblasts. BPTF knockdown leads to decreased c-MYC recruitment to DNA and changes in chromatin accessibility. In Bptf-null MEFs, BPTF is necessary for c-MYC-driven proliferation, G1-S progression and replication stress, but not for c-MYC-driven apoptosis. Bioinformatics analyses unveil that BPTF levels correlate positively with c-MYC-driven transcriptional signatures. In vivo, Bptf inactivation in pre-neoplastic pancreatic acinar cells significantly delays tumour development and extends survival. Our findings uncover BPTF as a crucial c-MYC co-factor required for its biological activity and suggest that the BPTF-c-MYC axis is a potential therapeutic target in cancer.es_ES
dc.description.sponsorshipWe thank the members of the Epithelial Carcinogenesis Group for valuable discussions;N. del Pozo for valuable contributions with animal experimentation; A. Picornell forvaluable contributions in an early phase of the project; M. Soengas for providing cells; M. Magnuson and C. V. Wright for providing mice; B. Amati, M. Beato, O. Fernandez-Capetillo, M. Serrano, G. Vicent and A. Sabo`for providing critical comments to prior versions of the manuscript. This work was supported, in part, by grants from Ministerio de Economıa y Competitividad, Madrid, Spain (SAF2007–60860, SAF2011–29530and ONCOBIO Consolider), Instituto de Salud Carlos III, Madrid, Spain (RTICCRD12/0036/0034), European Union Seventh Framework Programme (grant 256974), and an EIN/Roche-CNIO collaborative grant to F.X.R.; and grants from Instituto de Salud Carlos III (INTRASALUD PI12/00425 and RTICC RD12/0036/0037) to J.C.C.es_ES
dc.language.isoenges_ES
dc.publisherNATURE PUBLISHING GROUPes_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subject.meshAnimals es_ES
dc.subject.meshAntigens, Nuclear es_ES
dc.subject.meshCell Line es_ES
dc.subject.meshCell Proliferation es_ES
dc.subject.meshChromatin Assembly and Disassembly es_ES
dc.subject.meshDatabases, Factuales_ES
dc.subject.meshGene Expression Regulation, Neoplastic es_ES
dc.subject.meshGene Knockdown Techniques es_ES
dc.subject.meshHumans es_ES
dc.subject.meshMice es_ES
dc.subject.meshMice, Knockout es_ES
dc.subject.meshNerve Tissue Proteins es_ES
dc.subject.meshPancreatic Neoplasms es_ES
dc.subject.meshProto-Oncogene Proteins c-myc es_ES
dc.subject.meshTranscription Factors es_ES
dc.subject.meshCarcinogenesis es_ES
dc.titleBPTF is required for c-MYC transcriptional activity and in vivo tumorigenesises_ES
dc.typeArtículoes_ES
dc.rights.licenseAtribución-NoComercial-CompartirIgual 4.0 Internacional*
dc.identifier.pubmedID26729287es_ES
dc.format.volume7es_ES
dc.format.number1es_ES
dc.format.page10153es_ES
dc.identifier.doi10.1038/ncomms10153es_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.contributor.funderInstituto de Salud Carlos III - ISCIII (España)es_ES
dc.contributor.funderEuropean Union Seventh Framework Programmees_ES
dc.description.peerreviewedes_ES
dc.identifier.e-issn2041-1723es_ES
dc.relation.publisherversionhttps://doi.org/10.1038/ncomms10153.es_ES
dc.identifier.journalNature communicationses_ES
dc.repisalud.institucionCNIOes_ES
dc.repisalud.orgCNIOCNIO::Grupos de investigación::Grupo de Carcinogénesis Epiteliales_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/256974es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2007-60860es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2011-29530es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/ PI12/00425es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/RD12/0036/0037es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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This item is licensed under a: Atribución-NoComercial-CompartirIgual 4.0 Internacional