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Título
Evaluation of two rK39 dipstick tests, direct agglutination test, and indirect fluorescent antibody test for diagnosis of visceral leishmaniasis in a new epidemic site in highland Ethiopia
Autor(es)
Cañavate, Carmen ISCIII | Herrero, Merce | Nieto Martinez, Francisco Javier ISCIII | Cruz, Israel ISCIII | Chicharro, Carmen ISCIII | Aparicio, Pilar ISCIII | Mulugeta, Abate | Argaw, Daniel | Blackstock, Anna J | Alvar, Jorge | Bern, Caryn
Fecha de publicación
2011-01
Cita
Am J Trop Med Hyg. 2011;84(1):102-6
Idioma
Inglés
Tipo de documento
journal article
Resumen
We assessed the performance characteristics of two rK39 immunochromatographic tests, a direct agglutination test (DAT), and an indirect immunofluorescent antibody test (IFAT) in the site of a new epidemic of visceral leishmaniasis (VL) in northwestern Ethiopia. The study population was composed of 179 patients with suspected VL and 67 controls. The sensitivities of Kalazar Detect(®), DiaMed-IT Leish(®), DAT, and IFAT in 35 polymerase chain reaction-confirmed VL cases were 94.3%, 91.4%, 91.4%, and 100%, respectively, and the specificities were 98.5%, 94%, 98.5%, and 98.5%, respectively. In a Bayesian latent class analysis of all 246 specimens, the estimated sensitivities were 90.5%, 89%, 88.8%, and 96% for Kalazar Detect(®), DiaMed-IT Leish(®), DAT, and IFAT, respectively; DAT showed the highest estimated specificity (97.4%). Both rK39 immunochromatographic tests perform as well as DAT, and are suitable for VL diagnosis in first-level health centers in this area of Ethiopia.
Versión en línea
DOI
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