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dc.contributor.authorAguilar, Jose C. 
dc.contributor.authorPerez-Breña, Pilar 
dc.contributor.authorGarcía, M L
dc.contributor.authorCruz, N
dc.contributor.authorErdman, D D
dc.contributor.authorEchevarria, Juan Emilio
dc.identifier.citationJ Clin Microbiol. 2000 Mar;38(3):1191-5.es_ES
dc.descriptionCorrection. Detection and Identification of Human Parainfluenza Viruses 1, 2, 3, and 4 in Clinical Samples of Pediatric Patients by Multiplex Reverse Transcription-PCR. J Clin Microbiol 2000 Jul;38(7):2805.
dc.description.abstractWe describe a multiplex reverse transcription-PCR (m-RT-PCR) assay that is able to detect and differentiate all known human parainfluenza viruses (HPIVs). Serial dilution experiments with reference strains that compared cell culture isolation and m-RT-PCR showed sensitivities ranging from 0.0004 50% tissue culture infective dose (TCID(50)) for HPIV type 4B (HPIV-4B) to 32 TCID(50)s for HPIV-3. As few as 10 plasmids containing HPIV PCR products could be detected in all cases. When 201 nasopharyngeal aspirate specimens from pediatric patients hospitalized for lower respiratory illness were tested, m-RT-PCR assay detected 64 HPIVs (24 HPIV-3, 23 HPIV-1, 10 HPIV-4, and 7 HPIV-2), while only 42 of them (21 HPIV-1, 14 HPIV-3, 6 HPIV-2, and 1 HPIV-4 isolates) grew in cell culture. Our m-RT-PCR assay was more sensitive than either cell culture isolation or indirect immunofluorescence with monoclonal antibodies for the detection of HPIV infections. Also, HPIV-4 was more frequently detected than HPIV-2 in this study, suggesting that it may have been underestimated as a lower respiratory tract pathogen because of the insensitivity of cell culture.es_ES
dc.description.sponsorshipWe are very grateful to Francisco Pozo for help with the cloning experiments and Angel del Pozo for photographic work. This work was supported in part by “Fondo de Investigaciones Sanitarias” grant 98/0310 from the Spanish Ministry of Health.es_ES
dc.publisherAmerican Society for Microbiology (ASM) es_ES
dc.subject.meshAnimals es_ES
dc.subject.meshCell Line es_ES
dc.subject.meshChild es_ES
dc.subject.meshDNA Primers es_ES
dc.subject.meshDNA, Viral es_ES
dc.subject.meshDogs es_ES
dc.subject.meshFluorescent Antibody Technique, Indirect es_ES
dc.subject.meshHumans es_ES
dc.subject.meshInhalation es_ES
dc.subject.meshParainfluenza Virus 1, Human es_ES
dc.subject.meshParainfluenza Virus 2, Human es_ES
dc.subject.meshParainfluenza Virus 3, Human es_ES
dc.subject.meshRespirovirus Infections es_ES
dc.subject.meshRetrospective Studies es_ES
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction es_ES
dc.subject.meshRubulavirus es_ES
dc.subject.meshRubulavirus Infections es_ES
dc.subject.meshSensitivity and Specificity es_ES
dc.subject.meshTumor Cells, Cultured es_ES
dc.titleDetection and identification of human parainfluenza viruses 1, 2, 3, and 4 in clinical samples of pediatric patients by multiplex reverse transcription-PCRes_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución-NoComercial-CompartirIgual 4.0 Internacional*
dc.contributor.funderInstituto de Salud Carlos III 
dc.identifier.journalJournal of clinical microbiologyes_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.rights.accessRightsopen accesses_ES

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Atribución-NoComercial-CompartirIgual 4.0 Internacional
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