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dc.contributor.authorMartinez-Pinna, Roxana
dc.contributor.authorMadrigal-Matute, Julio
dc.contributor.authorTarín, Carlos
dc.contributor.authorBurillo, Elena
dc.contributor.authorEsteban-Salan, Margarita
dc.contributor.authorPastor-Vargas, Carlos
dc.contributor.authorLindholt, Jes S
dc.contributor.authorLopez, Juan Antonio 
dc.contributor.authorCalvo, Enrique 
dc.contributor.authorde Ceniga, Melina Vega
dc.contributor.authorMeilhac, Olivier
dc.contributor.authorEgido, Jesus
dc.contributor.authorBlanco-Colio, Luis M
dc.contributor.authorMichel, Jean-Baptiste
dc.contributor.authorMartin-Ventura, Jose L
dc.date.accessioned2019-05-23T12:21:02Z
dc.date.available2019-05-23T12:21:02Z
dc.date.issued2013-08
dc.identifier.citationArterioscler Thromb Vasc Biol. 2013; 33(8):2013-20es_ES
dc.identifier.issn1079-5642es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/7666
dc.description.abstractOBJECTIVE: To identify proteins related to intraluminal thrombus biological activities that could help to find novel pathological mechanisms and therapeutic targets for human abdominal aortic aneurysm (AAA). APPROACH AND RESULTS: Tissue-conditioned media from patients with AAA were analyzed by a mass spectrometry-based strategy using liquid chromatography coupled to tandem mass spectrometry. Global pathway analysis by Ingenuity software highlighted the presence of several circulating proteins, among them were proteins from the complement system. Complement C3 concentration and activation were assessed in plasma from AAA patients (small AAA, AAA diameter=3-5 cm and large AAA, AAA diameter >5 cm), showing decreased C3 levels and activation in large AAA patients. No association of a combination of single-nucleotide polymorphisms in complement genes between large and small AAA patients was observed. Intense extracellular C3 inmunostaining, along with C9, was observed in AAA thrombus. Analysis of C3 in AAA tissue homogenates and tissue-conditioned media showed increased levels of C3 in AAA thrombus, as well as proteolytic fragments (C3a/C3c/C3dg), suggesting its local deposition and activation. Finally, the functional role of local complement activation in polymorphonuclear (PMN) cell activation was tested, showing that C3 blockade by anti-C3 antibody was able to decrease thrombus-induced neutrophil chemotaxis and reactive oxygen species production. CONCLUSIONS: A decrease of systemic C3 concentration and activity in the later stages of AAA associated with local complement retention, consumption, and proteolysis in the thrombus could induce PMN chemotaxis and activation, playing a detrimental role in AAA progression.es_ES
dc.description.sponsorshipThe article has been supported by the European Community, Fighting Aneurysmal Disease project (FP-7, HEALTH F2-2008–200647), the Spanish MICIN (SAF2010/21852), Ministerio de Sanidad y Consumo, Instituto de Salud Carlos III, Red RIC (RD12/0042/00038), and biobancos (RD09/0076/00101), Fundación Lilly and Fundacion Pro Centro Nacional de Investigaciones.es_ES
dc.language.isoenges_ES
dc.publisherAmerican Heart Associationes_ES
dc.relation.isversionofPostprintes_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectaortic aneurysm, abdominales_ES
dc.subjectcomplement system proteinses_ES
dc.subjectinflammationes_ES
dc.subjectneutrophilses_ES
dc.subjectthrombosises_ES
dc.subject.meshAged es_ES
dc.subject.meshAged, 80 and over es_ES
dc.subject.meshAortic Aneurysm, Abdominal es_ES
dc.subject.meshAutoantibodies es_ES
dc.subject.meshChemotaxis es_ES
dc.subject.meshChromatography, Liquid es_ES
dc.subject.meshComplement C3 es_ES
dc.subject.meshComplement C9 es_ES
dc.subject.meshCulture Media, Conditioned es_ES
dc.subject.meshFemale es_ES
dc.subject.meshHumans es_ES
dc.subject.meshMale es_ES
dc.subject.meshMiddle Aged es_ES
dc.subject.meshNeutrophils es_ES
dc.subject.meshPolymorphism, Single Nucleotidees_ES
dc.subject.meshProteomics es_ES
dc.subject.meshReactive Oxygen Species es_ES
dc.subject.meshRisk Factors es_ES
dc.subject.meshTandem Mass Spectrometry es_ES
dc.subject.meshThrombosis es_ES
dc.titleProteomic analysis of intraluminal thrombus highlights complement activation in human abdominal aortic aneurysmses_ES
dc.typeArtículoes_ES
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.identifier.pubmedID23702661es_ES
dc.format.volume33es_ES
dc.format.number8es_ES
dc.format.page2013-20es_ES
dc.identifier.doi10.1161/ATVBAHA.112.301191es_ES
dc.contributor.funderEuropean Commissiones_ES
dc.contributor.funderMinisterio de Ciencia e Innovación (España)es_ES
dc.contributor.funderMinisterio de Sanidad y Consumo (España)es_ES
dc.contributor.funderInstituto de Salud Carlos III - ISCIIIes_ES
dc.contributor.funderFundación ProCNICes_ES
dc.contributor.funderFundación Lillyes_ES
dc.description.peerreviewedes_ES
dc.identifier.e-issn1524-4636es_ES
dc.relation.publisherversionhttps://doi.org/10.1161/ATVBAHA.112.301191es_ES
dc.identifier.journalArteriosclerosis, thrombosis, and vascular biologyes_ES
dc.repisalud.orgCNICCNIC::Unidades técnicas::Proteómica / Metabolómicaes_ES
dc.repisalud.institucionCNICes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/200647/EUes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2010/21852es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/RD12/0042/00038es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/RD09/0076/00101es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
This item is licensed under a: Attribution-NonCommercial-NoDerivatives 4.0 Internacional