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dc.contributor.authorKao, Damian
dc.contributor.authorFelix, Daniel 
dc.contributor.authorAboobaker, Aziz
dc.date.accessioned2019-05-14T13:10:20Z
dc.date.available2019-05-14T13:10:20Z
dc.date.issued2013-11-16
dc.identifier.citationBMC Genomics. 2013; 14(1):797es_ES
dc.identifier.issn1471-2164es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/7572
dc.description.abstractBACKGROUND: Planarians can regenerate entire animals from a small fragment of the body. The regenerating fragment is able to create new tissues and remodel existing tissues to form a complete animal. Thus different fragments with very different starting components eventually converge on the same solution. In this study, we performed an extensive RNA-seq time-course on regenerating head and tail fragments to observe the differences and similarities of the transcriptional landscape between head and tail fragments during regeneration. RESULTS: We have consolidated existing transcriptomic data for S. mediterranea to generate a high confidence set of transcripts for use in genome wide expression studies. We performed a RNA-seq time-course on regenerating head and tail fragments from 0 hours to 3 days. We found that the transcriptome profiles of head and tail regeneration were very different at the start of regeneration; however, an unexpected convergence of transcriptional profiles occurred at 48 hours when head and tail fragments are still morphologically distinct. By comparing differentially expressed transcripts at various time-points, we revealed that this divergence/convergence pattern is caused by a shared regulatory program that runs early in heads and later in tails.Additionally, we also performed RNA-seq on smed-prep(RNAi) tail fragments which ultimately fail to regenerate anterior structures. We find the gene regulation program in response to smed-prep(RNAi) to display the opposite regulatory trend compared to the previously mentioned share regulatory program during regeneration. Using annotation data and comparative approaches, we also identified a set of approximately 4,800 triclad specific transcripts that were enriched amongst the genes displaying differential expression during the regeneration time-course. CONCLUSION: The regeneration transcriptome of head and tail regeneration provides us with a rich resource for investigating the global expression changes that occurs during regeneration. We show that very different regenerative scenarios utilize a shared core regenerative program. Furthermore, our consolidated transcriptome and annotations allowed us to identity triclad specific transcripts that are enriched within this core regulatory program. Our data support the hypothesis that both conserved aspects of animal developmental programs and recent evolutionarily innovations work in concert to control regeneration.es_ES
dc.language.isoenges_ES
dc.publisherBMCes_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.meshAnimals es_ES
dc.subject.meshCluster Analysis es_ES
dc.subject.meshExpressed Sequence Tags es_ES
dc.subject.meshHead es_ES
dc.subject.meshHelminth Proteins es_ES
dc.subject.meshOpen Reading Frames es_ES
dc.subject.meshPlanarians es_ES
dc.subject.meshRNA Interference es_ES
dc.subject.meshRNA, Helminth es_ES
dc.subject.meshRNA, Messenger es_ES
dc.subject.meshTail es_ES
dc.subject.meshGene Expression Regulation es_ES
dc.subject.meshRegeneration es_ES
dc.subject.meshTranscriptome es_ES
dc.titleThe planarian regeneration transcriptome reveals a shared but temporally shifted regulatory program between opposing head and tail scenarioses_ES
dc.typeArtículoes_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID24238224es_ES
dc.format.volume14es_ES
dc.format.number1es_ES
dc.format.page797es_ES
dc.identifier.doi10.1186/1471-2164-14-797es_ES
dc.description.peerreviewedes_ES
dc.identifier.e-issn1471-2164es_ES
dc.relation.publisherversionhttps://doi.org/10.1186/1471-2164-14-797es_ES
dc.identifier.journalBMC genomicses_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Antiguos CNICes_ES
dc.repisalud.institucionCNICes_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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Atribución 4.0 Internacional
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