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dc.contributor.authorRibas, L.
dc.contributor.authorCrespo, B.
dc.contributor.authorSánchez-Baizán, N.
dc.contributor.authorXavier, D.
dc.contributor.authorKuhl, H.
dc.contributor.authorRodriguez, Jose Manuel 
dc.contributor.authorDíaz, N.
dc.contributor.authorBoltañá, S.
dc.contributor.authorMacKenzie, S.
dc.contributor.authorMorán, F.
dc.contributor.authorZanuy, S.
dc.contributor.authorGómez, A.
dc.contributor.authorPiferrer, F.
dc.date.accessioned2019-05-09T09:19:43Z
dc.date.available2019-05-09T09:19:43Z
dc.date.issued2019-03-27
dc.identifier.citationMar Biotechnol (NY). 2019, Mar 27es_ES
dc.identifier.issn1436-2228es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/7559
dc.description.abstractThe European sea bass is one of the most important cultured fish in Europe and has a marked sexual growth dimorphism in favor of females. It is a gonochoristic species with polygenic sex determination, where a combination between still undifferentiated genetic factors and environmental temperature determines sex ratios. The molecular mechanisms responsible for gonadal sex differentiation are still unknown. Here, we sampled fish during the gonadal developmental period (110 to 350 days post fertilization, dpf), and performed a comprehensive transcriptomic study by using a species-specific microarray. This analysis uncovered sex-specific gonadal transcriptomic profiles at each stage of development, identifying larger number of differentially expressed genes in ovaries when compared to testis. The expression patterns of 54 reproduction-related genes were analyzed. We found that hsd17β10 is a reliable marker of early ovarian differentiation. Further, three genes, pdgfb, snx1, and nfy, not previously related to fish sex differentiation, were tightly associated with testis development in the sea bass. Regarding signaling pathways, lysine degradation, bladder cancer, and NOD-like receptor signaling were enriched for ovarian development while eight pathways including basal transcription factors and steroid biosynthesis were enriched for testis development. Analysis of the transcription factor abundance showed an earlier increase in females than in males. Our results show that, although many players in the sex differentiation pathways are conserved among species, there are peculiarities in gene expression worth exploring. The genes identified in this study illustrate the diversity of players involved in fish sex differentiation and can become potential biomarkers for the management of sex ratios in the European sea bass and perhaps other cultured species.es_ES
dc.language.isoenges_ES
dc.publisherSpringer es_ES
dc.type.hasVersionSMURes_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectAquaculturees_ES
dc.subjectGenomicses_ES
dc.subjectGonadses_ES
dc.subjectReproductiones_ES
dc.subjectSex differentiationes_ES
dc.subjectTranscriptomicses_ES
dc.titleCharacterization of the European Sea Bass (Dicentrarchus labrax) Gonadal Transcriptome During Sexual Development [Pre-print]es_ES
dc.typepreprintes_ES
dc.rights.licenseAtribución-NoComercial-CompartirIgual 4.0 Internacional*
dc.identifier.pubmedID30919121es_ES
dc.identifier.doi10.1007/s10126-019-09886-xes_ES
dc.description.peerreviewedNoes_ES
dc.identifier.e-issn1436-2236es_ES
dc.relation.publisherversionhttps://doi.org/10.1007/s10126-019-09886-xes_ES
dc.identifier.journalMarine Biotechnologyes_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Proteómica cardiovasculares_ES
dc.repisalud.institucionCNICes_ES
dc.rights.accessRightsopen accesses_ES


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Atribución-NoComercial-CompartirIgual 4.0 Internacional
Este Item está sujeto a una licencia Creative Commons: Atribución-NoComercial-CompartirIgual 4.0 Internacional