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dc.contributor.authorVillarroya-Beltri, Carolina 
dc.contributor.authorGutierrez-Vazquez, Cristina 
dc.contributor.authorSanchez-Cabo, Fatima 
dc.contributor.authorPerez-Hernandez, Daniel 
dc.contributor.authorVazquez, Jesus 
dc.contributor.authorMartin-Cofreces, Noa
dc.contributor.authorMartinez-Herrera, Dannys Jorge
dc.contributor.authorPascual-Montano, Alberto
dc.contributor.authorMittelbrunn, Maria 
dc.contributor.authorSanchez-Madrid, Francisco 
dc.date.accessioned2019-05-07T11:37:49Z
dc.date.available2019-05-07T11:37:49Z
dc.date.issued2013
dc.identifier.citationNat Commun. 2013; 4:2980es_ES
dc.identifier.issn2041-1723es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/7548
dc.description.abstractExosomes are released by most cells to the extracellular environment and are involved in cell-to-cell communication. Exosomes contain specific repertoires of mRNAs, microRNAs (miRNAs) and other non-coding RNAs that can be functionally transferred to recipient cells. However, the mechanisms that control the specific loading of RNA species into exosomes remain unknown. Here we describe sequence motifs present in miRNAs that control their localization into exosomes. The protein heterogeneous nuclear ribonucleoprotein A2B1 (hnRNPA2B1) specifically binds exosomal miRNAs through the recognition of these motifs and controls their loading into exosomes. Moreover, hnRNPA2B1 in exosomes is sumoylated, and sumoylation controls the binding of hnRNPA2B1 to miRNAs. The loading of miRNAs into exosomes can be modulated by mutagenesis of the identified motifs or changes in hnRNPA2B1 expression levels. These findings identify hnRNPA2B1 as a key player in miRNA sorting into exosomes and provide potential tools for the packaging of selected regulatory RNAs into exosomes and their use in biomedical applications.es_ES
dc.description.sponsorshipThis work was supported by grants SAF2011-25834, ERC-2011-AdG, BIO2009_07990, BIO2010-17527, COST-Action BM1202, P210/BMD-2305 and Cardiovascular Network RD12-0042-0056 from Instituto Salud Carlos III. C.V.B. was supported by FPU program (Spanish Ministry of Education). M.M. was supported by the Instituto de Salud Carlos III, C.G.-V. by the Comunidad de Madrid and D.J.M.H. by Programa La Caixa.es_ES
dc.language.isoenges_ES
dc.publisherNature Publishing Groupes_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subject.meshAmino Acid Motifs es_ES
dc.subject.meshCell Communication es_ES
dc.subject.meshExosomes es_ES
dc.subject.meshGene Silencing es_ES
dc.subject.meshHeterogeneous-Nuclear Ribonucleoprotein Group A-B es_ES
dc.subject.meshHumans es_ES
dc.subject.meshJurkat Cells es_ES
dc.subject.meshLeukocytes, Mononuclear es_ES
dc.subject.meshMicroRNAs es_ES
dc.subject.meshMutagenesis es_ES
dc.subject.meshMutagenesis, Site-Directed es_ES
dc.subject.meshMutation es_ES
dc.subject.meshOligonucleotide Array Sequence Analysis es_ES
dc.subject.meshProtein Binding es_ES
dc.subject.meshSumoylation es_ES
dc.titleSumoylated hnRNPA2B1 controls the sorting of miRNAs into exosomes through binding to specific motifses_ES
dc.typeArtículoes_ES
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.identifier.pubmedID24356509es_ES
dc.format.volume4es_ES
dc.format.number1es_ES
dc.format.page2980es_ES
dc.identifier.doi10.1038/ncomms3980es_ES
dc.contributor.funderEuropean Commissiones_ES
dc.contributor.funderEuropean Research Counciles_ES
dc.contributor.funderInstituto de Salud Carlos III - ISCIIIes_ES
dc.contributor.funderMinisterio de Educación (España)es_ES
dc.contributor.funderComunidad de Madrides_ES
dc.contributor.funderFundación La Caixaes_ES
dc.description.peerreviewedes_ES
dc.identifier.e-issn2041-1723es_ES
dc.relation.publisherversionhttps://doi.org/10.1038/ncomms3980es_ES
dc.identifier.journalNature communicationses_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Comunicación Intercelular en la Respuesta Inflamatoriaes_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Proteómica cardiovasculares_ES
dc.repisalud.orgCNICCNIC::Unidades técnicas::Bioinformáticaes_ES
dc.repisalud.institucionCNICes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/294340/EUes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2011-25834es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/BIO2009-07990es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/BIO2010-17527es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/RD12-0042-0056es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
This item is licensed under a: Attribution-NonCommercial-NoDerivatives 4.0 Internacional