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dc.contributor.author | Zotes, Teresa M | |
dc.contributor.author | Arias, Cristina | |
dc.contributor.author | Fuster, Jose J. | |
dc.contributor.author | Spada, Roberto | |
dc.contributor.author | Pérez-Yagüe, Sonia | |
dc.contributor.author | Hirsch, Emilio | |
dc.contributor.author | Wymann, Matthias | |
dc.contributor.author | Carrera, Ana C | |
dc.contributor.author | Andres, Vicente | |
dc.contributor.author | Barber, Domingo F | |
dc.date.accessioned | 2019-05-07T11:08:51Z | |
dc.date.available | 2019-05-07T11:08:51Z | |
dc.date.issued | 2013 | |
dc.identifier.citation | PLoS One. 2013; 8(8):e72674 | es_ES |
dc.identifier.issn | 1932-6203 | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/7546 | |
dc.description.abstract | Atherosclerosis is an inflammatory disease regulated by infiltrating monocytes and T cells, among other cell types. Macrophage recruitment to atherosclerotic lesions is controlled by monocyte infiltration into plaques. Once in the lesion, macrophage proliferation in situ, apoptosis, and differentiation to an inflammatory (M1) or anti-inflammatory phenotype (M2) are involved in progression to advanced atherosclerotic lesions. We studied the role of phosphoinositol-3-kinase (PI3K) p110γ in the regulation of in situ apoptosis, macrophage proliferation and polarization towards M1 or M2 phenotypes in atherosclerotic lesions. We analyzed atherosclerosis development in LDLR(-/-)p110γ(+/-) and LDLR(-/-)p110γ(-/-) mice, and performed expression and functional assays in tissues and primary cells from these and from p110γ(+/-) and p110γ(-/-) mice. Lack of p110γ in LDLR(-/-) mice reduces the atherosclerosis burden. Atherosclerotic lesions in fat-fed LDLR(-/-)p110γ(-/-) mice were smaller than in LDLR(-/-)p110γ(+/-) controls, which coincided with decreased macrophage proliferation in LDLR(-/-)p110γ(-/-) mouse lesions. This proliferation defect was also observed in p110γ(-/-) bone marrow-derived macrophages (BMM) stimulated with macrophage colony-stimulating factor (M-CSF), and was associated with higher intracellular cyclic adenosine monophosphate (cAMP) levels. In contrast, T cell proliferation was unaffected in LDLR(-/-)p110γ(-/-) mice. Moreover, p110γ deficiency did not affect macrophage polarization towards the M1 or M2 phenotypes or apoptosis in atherosclerotic plaques, or polarization in cultured BMM. Our results suggest that higher cAMP levels and the ensuing inhibition of macrophage proliferation contribute to atheroprotection in LDLR(-/-) mice lacking p110γ. Nonetheless, p110γ deletion does not appear to be involved in apoptosis, in macrophage polarization or in T cell proliferation. | es_ES |
dc.description.sponsorship | This work was supported by the Spanish Ministry of Science and Innovation (SAF-2007-60498, SAF-2008-00471, SAF-2011-23639 to DFB, SAF201016044 to VA, AP2007-01711 to TMZ, BES-2009-016547 to RS), the Research Network in Inflammation and Rheumatic Diseases (RIER) of the ISCIII-MSPS Cooperative Research Thematic Network program (RD08/0075/0015 to DFB), the Madrid Regional Government (200520M040 to DFB) and the Thematic Research Network in Cardiovascular Diseases (RECAVA; RD06/0014/0021 to VA). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Public Library of Science (PLOS) | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject.mesh | Animals | es_ES |
dc.subject.mesh | Apoptosis | es_ES |
dc.subject.mesh | Atherosclerosis | es_ES |
dc.subject.mesh | Class Ib Phosphatidylinositol 3-Kinase | es_ES |
dc.subject.mesh | Cyclic AMP | es_ES |
dc.subject.mesh | Macrophages | es_ES |
dc.subject.mesh | Mice | es_ES |
dc.subject.mesh | Mice, Knockout | es_ES |
dc.subject.mesh | Receptors, LDL | es_ES |
dc.subject.mesh | Cell Proliferation | es_ES |
dc.title | PI3K p110γ deletion attenuates murine atherosclerosis by reducing macrophage proliferation but not polarization or apoptosis in lesions | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 23991137 | es_ES |
dc.format.volume | 8 | es_ES |
dc.format.number | 8 | es_ES |
dc.format.page | e72674 | es_ES |
dc.identifier.doi | 10.1371/journal.pone.0072674 | es_ES |
dc.contributor.funder | Ministerio de Ciencia e Innovación (España) | |
dc.contributor.funder | Instituto de Salud Carlos III | |
dc.contributor.funder | Comunidad de Madrid (España) | |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 1932-6203 | es_ES |
dc.relation.publisherversion | https://doi.org/10.1371/journal.pone.0072674 | es_ES |
dc.identifier.journal | PloS one | es_ES |
dc.repisalud.orgCNIC | CNIC::Grupos de investigación::Fisiopatología Cardiovascular Molecular y Genética | es_ES |
dc.repisalud.institucion | CNIC | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/SAF-2007-60498 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/SAF-2008-00471 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/SAF-2011-23639 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/SAF-2010-16044 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/BES-2009-016547 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/RD08/0075/0015 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/RD06/0014/0021 | es_ES |
dc.rights.accessRights | open access | es_ES |