Mostrar el registro sencillo del ítem

dc.contributor.authorRubio Muñoz, Jose Miguel 
dc.contributor.authorBenito, Agustin 
dc.contributor.authorBerzosa, Pedro 
dc.contributor.authorRoche, Jesus 
dc.contributor.authorPuente, S
dc.contributor.authorSubirats, M
dc.contributor.authorLópez-Vélez, R
dc.contributor.authorGarcia, L 
dc.contributor.authorAlvar, Jorge 
dc.date.accessioned2019-05-06T11:05:13Z
dc.date.available2019-05-06T11:05:13Z
dc.date.issued1999-10
dc.identifier.citationJ Clin Microbiol. 1999 Oct;37(10):3260-4es_ES
dc.identifier.issn0095-1137es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/7539
dc.description.abstractThe use of a new PCR-based method for the diagnosis of malaria in the Spanish Malaria Reference Laboratory has promoted an increase in confirmed cases of malaria. From August 1997 to July 1998, a total of 192 whole-blood samples and 71 serum samples from 168 patients were received from the hospitals of the Spanish National Health System. Most of the patients came from west-central African countries (85%). This molecular method showed more sensitivity and specificity than microscopy, detecting 12.4% more positive samples than microscopy and 13% of mixed infections undetectable by Giemsa stain. Plasmodium falciparum was the main species detected, with 68% of the total positive malaria cases, followed by Plasmodium malariae (29%), Plasmodium vivax (14%), and Plasmodium ovale (7%), including mixed infections in all cases. This report consists of the first wide, centralized survey of malaria surveillance in Spain. The reference laboratory conducted the analysis of all imported cases in order to detect trends in acquisition. The use of a seminested multiplex PCR permitted confirmation of the origins of the infections and the Plasmodium species involved and confirmation of the effectiveness of drug treatments. This PCR also allowed the detection of the presence in Spain of primaquine-tolerant P. vivax strains from west-central Africa, as well as the detection of a P. falciparum infection induced by transfusion.es_ES
dc.description.sponsorshipThis work was supported by the Fondo de Investigaciones Sanitarias (FIS) (contract number 96/0216) and the Spanish Agency of International Cooperation (AECI). J. M. Rubio was granted a postdoctoral fellowship from the Comunidad Autónoma de Madrid, Madrid, Spain. J. Alvar was supported by a B.A.E. from the FIS (contract number 99/5038) and by the Christ’s College, University of Cambridge, Cambridge, United Kingdomes_ES
dc.language.isoenges_ES
dc.publisherASM International es_ES
dc.type.hasVersionAMes_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subject.meshChild, Preschool es_ES
dc.subject.meshFemale es_ES
dc.subject.meshHumans es_ES
dc.subject.meshMalaria es_ES
dc.subject.meshMale es_ES
dc.subject.meshMiddle Aged es_ES
dc.subject.meshPolymerase Chain Reaction es_ES
dc.subject.meshSpain es_ES
dc.titleUsefulness of seminested multiplex PCR in surveillance of imported malaria in Spaines_ES
dc.typepreprintes_ES
dc.rights.licenseAtribución-NoComercial-CompartirIgual 4.0 Internacional*
dc.identifier.pubmedID10488189es_ES
dc.format.volume37es_ES
dc.format.number10es_ES
dc.format.page3260-4es_ES
dc.contributor.funderInstituto de Salud Carlos III 
dc.contributor.funderAgencia Española de Cooperación Internacional para el Desarrollo 
dc.contributor.funderComunidad de Madrid (España) 
dc.identifier.journalJournal of clinical microbiologyes_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.centroISCIII::Centro Nacional de Medicina Tropicales_ES
dc.repisalud.institucionISCIIIes_ES
dc.rights.accessRightsopen accesses_ES


Ficheros en el ítem

Acceso Abierto
Thumbnail

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem

Atribución-NoComercial-CompartirIgual 4.0 Internacional
Este Item está sujeto a una licencia Creative Commons: Atribución-NoComercial-CompartirIgual 4.0 Internacional