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dc.contributor.authorCastaño, Julio
dc.contributor.authorBueno, Clara
dc.contributor.authorJiménez-Delgado, Senda
dc.contributor.authorRoca-Ho, Heleia
dc.contributor.authorFraga, Mario F
dc.contributor.authorFernandez, Agustín F
dc.contributor.authorNakanishi, Mahito
dc.contributor.authorTorres-Ruiz Raul, Raúl 
dc.contributor.authorRodriguez Perales, Sandra 
dc.contributor.authorMenéndez, Pablo
dc.date.accessioned2019-04-02T11:45:11Z
dc.date.available2019-04-02T11:45:11Z
dc.date.issued2017
dc.identifier.citationStem Cell Res. 2017;21:137-140es_ES
dc.identifier.issn18735061es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/7430
dc.description.abstractWe report the generation-characterization of a fetal liver (FL) B-cell progenitor (BCP)-derived human induced pluripotent stem cell (hiPSC) line CRISPR/Cas9-edited to carry/express a single copy of doxycycline-inducible Cas9 gene in the "safe locus" AAVS1 (iCas9-FL-BCP-hiPSC). Gene-edited iPSCs remained pluripotent after CRISPR/Cas9 genome-edition. Correct genomic integration of a unique copy of Cas9 was confirmed by PCR and Southern blot. Cas9 was robustly and specifically expressed on doxycycline exposure. T7-endonuclease assay demonstrated that iCas9 induces robust gene-edition when gRNAs against hematopoietic transcription factors were tested. This iCas9-FL-BCP-hiPSC will facilitate gene-editing approaches for studies on developmental biology, drug screening and disease modeling.es_ES
dc.language.isoenges_ES
dc.type.hasVersionVoRes_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.meshB-Lymphocytes es_ES
dc.subject.meshBacterial Proteins es_ES
dc.subject.meshCRISPR-Associated Protein 9 es_ES
dc.subject.meshCRISPR-Cas Systems es_ES
dc.subject.meshEndonucleases es_ES
dc.subject.meshGene Editing es_ES
dc.subject.meshHumans es_ES
dc.subject.meshInduced Pluripotent Stem Cells es_ES
dc.subject.meshDependovirus es_ES
dc.subject.meshGene Expression es_ES
dc.subject.meshGenetic Loci es_ES
dc.titleGeneration and characterization of a human iPSC cell line expressing inducible Cas9 in the "safe harbor" AAVS1 locuses_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID28677529es_ES
dc.format.volume21es_ES
dc.format.page137-140es_ES
dc.identifier.doi10.1016/j.scr.2017.04.011es_ES
dc.description.peerreviewedes_ES
dc.identifier.e-issn1876-7753es_ES
dc.relation.publisherversionhttps://doi.org/10.1016/j.scr.2017.04.011.es_ES
dc.identifier.journalStem cell researches_ES
dc.repisalud.institucionCNIOes_ES
dc.repisalud.orgCNIOCNIO::Unidades técnicas::Unidad de Citogenética Moleculares_ES
dc.rights.accessRightsopen accesses_ES


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Atribución 4.0 Internacional
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