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dc.contributor.authorAlcolea, Pedro J
dc.contributor.authorAlonso, Ana
dc.contributor.authorGomez, Manuel J 
dc.contributor.authorPostigo, Marina
dc.contributor.authorMolina, Ricardo 
dc.contributor.authorJimenez, Maribel 
dc.contributor.authorLarraga, Vicente
dc.date.accessioned2019-04-02T10:15:42Z
dc.date.available2019-04-02T10:15:42Z
dc.date.issued2014-10-03
dc.identifier.citationBMC Genomics. 2014 Oct 3;15:849.es_ES
dc.identifier.issn1471-2164es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/7425
dc.description.abstractBACKGROUND: Leishmania infantum is the etiological agent of zoonotical visceral leishmaniasis in the Mediterranean basin. A recent outbreak in humans has been recently reported in central Spain. Leishmania spp. parasites are transmitted to the mammalian host by the bite of sand flies. The primary vector of L. infantum in Spain is Phlebotomus perniciosus. For decades, research on these parasites has involved the axenic culture model of the promastigote stage including gene expression profiling studies performed in the post-genome era. Unlike the controversial axenic culturing of amastigotes, promastigote cultures are generally accepted and used, although with the precaution of avoiding excessive culture passage.The primary objective of this differentiation study is to compare the gene expression profiles of promastigotes isolated from the foregut of the sand fly and amastigotes. For this purpose, P. perniciosus sand flies were infected with L. infantum and differentiated promastigotes were extracted by dissection of the foreguts. Shotgun DNA microarray hybridization analyses allowed for transcriptome comparison of these promastigotes with amastigotes obtained by infection of the U937 cell line. The results have been compared with those described in published expression analyses using axenic promastigotes. RESULTS: A total of 277 up-regulated genes were found through this hybridization experiment. The comparison of these particular results with published gene expression profile analyses performed using the same experimental procedure to study cultured promastigotes in stationary phase versus amastigotes revealed considerable differences (approximately 95% of the up-regulated genes were different). We found that the up-regulation rate is lower in amastigotes than in sand fly-derived promastigotes, which is in agreement with the over-expression of genes involved in gene expression regulation and signaling in those promastigote populations. CONCLUSIONS: The up-regulation rate is lower in intracellular amastigotes than in promastigotes obtained from the sand fly gut. This was also reported by us using the promastigote culture model and is an evidence for the hypothesis of promastigote preadaptation towards life in the intracellular environment. Regarding transcript abundance, the set of differentially regulated genes is notably different when using promastigotes from the sand fly foregut instead of axenic cultures.es_ES
dc.description.sponsorshipThe cost of this work has been partially defrayed by the grants AGL2010-21806-C02-01 (Spanish Ministry of Science, MICINN), RICET (RETICS-FIS, FEDER) collaborative network grant and 050204100014 (Fundación Ramón Areces), OTT code 20100338.es_ES
dc.language.isoenges_ES
dc.publisherBioMed Central (BMC) es_ES
dc.type.hasVersionVoRes_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.meshAnimals es_ES
dc.subject.meshCluster Analysis es_ES
dc.subject.meshGene Expression Profiling es_ES
dc.subject.meshGene Expression Regulation es_ES
dc.subject.meshHumans es_ES
dc.subject.meshIntestinal Mucosa es_ES
dc.subject.meshLeishmania infantum es_ES
dc.subject.meshLife Cycle Stages es_ES
dc.subject.meshNucleic Acid Hybridization es_ES
dc.subject.meshOligonucleotide Array Sequence Analysis es_ES
dc.subject.meshPhagocytes es_ES
dc.subject.meshPhlebotomus es_ES
dc.subject.meshRNA, Messenger es_ES
dc.subject.meshReal-Time Polymerase Chain Reaction es_ES
dc.subject.meshU937 Cells es_ES
dc.subject.meshUp-Regulation es_ES
dc.titleStage-specific differential gene expression in Leishmania infantum: from the foregut of Phlebotomus perniciosus to the human phagocytees_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID25281593es_ES
dc.format.volume15es_ES
dc.format.number1es_ES
dc.format.page849es_ES
dc.identifier.doi10.1186/1471-2164-15-849es_ES
dc.contributor.funderMinisterio de Ciencia e Innovación (España) 
dc.contributor.funderFundación Ramón Areces 
dc.description.peerreviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.1186/1471-2164-15-849es_ES
dc.identifier.journalBMC genomicses_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/AGL2010-21806-C02-01es_ES
dc.rights.accessRightsopen accesses_ES


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Atribución 4.0 Internacional
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