Please use this identifier to cite or link to this item:http://hdl.handle.net/20.500.12105/7365
XTEND: Extending the depth of field in cryo soft X-ray tomography
Sci Rep. 2017 Apr;7:45808
We have developed a new data collection method and processing framework in full field cryo soft X-ray tomography to computationally extend the depth of field (DOF) of a Fresnel zone plate lens. Structural features of 3D-reconstructed eukaryotic cells that are affected by DOF artifacts in standard reconstruction are now recovered. This approach, based on focal series projections, is easily applicable with closed expressions to select specific data acquisition parameters.
Files in this item