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dc.contributor.authorNieto, Amelia
dc.contributor.authorPozo Sánchez, Francisco 
dc.contributor.authorVidal-García, Matxalen
dc.contributor.authorOmeñaca, Manuel
dc.contributor.authorCasas, Inmaculada 
dc.contributor.authorFalcon, Ana
dc.identifier.citationFront Microbiol. 2017 Apr;8:575.es_ES
dc.description.abstractSeveral amino acid changes have been previously implicated in adaptation of avian influenza viruses to human hosts, among them the D701N change in the PB2 polymerase subunit that also is the main determinant of avian virus pathogenesis in animal models. However, previous studies using recombinant viruses did not provide conclusive information of the contribution of this PB2 residue to pathogenicity in human influenza virus strains. We identified this mutation in an A(H1N1)pdm09-like human influenza virus isolated from an infected patient with pneumonia and acute respiratory failure, admitted to the intensive care unit. An exhaustive search has revealed PB2-D701 as a highly conserved position in all available H1N1 human virus sequences in NCBI database, showing a very low prevalence of PB2-D701N change. Presence of PB2-701N amino acid correlates with severe or fatal outcome in those scarce cases with known disease outcome of the infection. In these patients, the residue PB2-701N may contribute to pathogenicity as it was previously reported in humans infected with avian viruses. This study helps to clarify a debate that has arisen regarding the role of PB2-D701N in human influenza virus pathogenicity.es_ES
dc.description.sponsorshipWe are indebted to J. Ortín and P. Gastaminza for their critiques of the manuscript. We gratefully acknowledge the technical assistance of N. Zamarreño and the editorial assistance of C. Mark. We thank members of the Spanish Influenza Surveillance System who work on the identification and declaration of patients during influenza seasons. We also thank J. C. Oliveros for deepsequencing analysis assistance.es_ES
dc.publisherFrontiers Mediaes_ES
dc.relation.isversionofPublisher's versiones_ES
dc.subjectD701N mutationes_ES
dc.subjectPB2 subunites_ES
dc.subjectAdaptation of avian viruseses_ES
dc.subjectInfluenza viruses_ES
dc.titleIdentification of Rare PB2-D701N Mutation from a Patient with Severe Influenza: Contribution of the PB2-D701N Mutation to the Pathogenicity of Human Influenzaes_ES
dc.rights.licenseAtribución-NoComercial-CompartirIgual 4.0 Internacional*
dc.contributor.funderMinisterio de Economía (España)
dc.contributor.funderInstituto de Salud Carlos III - ISCIII
dc.identifier.journalFrontiers in microbiologyes_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES

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Atribución-NoComercial-CompartirIgual 4.0 Internacional
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