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dc.contributor.authorGarcía, Marcial
dc.contributor.authorGórgolas, Miguel
dc.contributor.authorCabello, Alfonso
dc.contributor.authorEstrada, Vicente
dc.contributor.authorLigos, Jose M. 
dc.contributor.authorFernández-Guerrero, Manuel
dc.contributor.authorBarros, Carlos
dc.contributor.authorLópez-Bernaldo, Juan Carlos
dc.contributor.authorDe La Hera, Francisco Javier
dc.contributor.authorMontoya, María 
dc.contributor.authorBenito, José Miguel
dc.contributor.authorRallón, Norma
dc.identifier.citationSci Rep. 2017; 7(1):16799es_ES
dc.description.abstractHIV latency is the main barrier to HIV eradication. Peripheral T follicular helper (pTfh) cells have a prominent role in HIV persistence. Herein, we analyzed the HIV reservoir size within memory CD4+ T-cell subsets in patients with HIV replication control. Twenty HIV-infected patients with suppressed HIV replication were included, with 10 elite controllers (EC) and 10 treated (TX) individuals. The HIV reservoir size was analyzed in resting memory CD4+ T-cells (Trm), pTfh, and non-pTfh cells using an ultrasensitive digital-droplet-PCR assay. Inter-group and intra-group differences were tested using non-parametric tests. Compared with the TX patients, the EC patients had smaller HIV reservoir not only in Trm but also in pTfh and non-pTfh subsets of memory CD4+ T-cells. The largest differences were observed in pTfh cells (p = 0.025). The pTfh and non-pTfh cells harbored similar levels of HIV-DNA in the EC (p = 0.60) and TX patients (p = 0.17); however, the contribution to HIV-DNA levels in memory CD4+ T-cells varied among the pTfh and non-pTfh subsets in both groups of patients. The EC patients showed smaller HIV reservoir in memory CD4+ cells, especially in the pTfh subset, a population of cells with a pivotal role in the antiviral immune response, suggesting a potential link between low levels of infection in pTfh cells and the ability of the EC patients to spontaneously control HIV replication.es_ES
dc.description.sponsorshipThis study has been funded by project CP14/00198 integrated in the State Plan for Scientific and Technical Research and Innovation and co-funded by ISCIII-Sub-Directorate General for Research Assessment and Promotion and European Regional Development Fund (ERDF). N Rallón is a Miguel Servet investigator from the Spanish Carlos III Institute of Health (ISCIII), grant CP14/00198, Madrid, Spain. M García is a predoctoral student co-funded by CP14/00198 project and the Intramural Research Scholarship from IIS-FJD.es_ES
dc.publisherNature Publishing Groupes_ES
dc.relation.isversionofPublisher's versiones_ES
dc.titlePeripheral T follicular helper Cells Make a Difference in HIV Reservoir Size between Elite Controllers and Patients on Successful cARTes_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.contributor.funderInstituto de Salud Carlos III - ISCIII
dc.contributor.funderEuropean Regional Development Fund (ERDF/FEDER)
dc.contributor.funderInstituto de Investigación Sanitaria de la Fundación Jiménez Díaz
dc.identifier.journalScientific reportses_ES
dc.repisalud.orgCNICCNIC::Unidades técnicas::Celómicaes_ES

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