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dc.contributor.authorBinek, Aleksandra 
dc.contributor.authorFernandez-Jimenez, Rodrigo 
dc.contributor.authorJorge, Inmaculada 
dc.contributor.authorCamafeita, Emilio 
dc.contributor.authorLopez, Juan Antonio 
dc.contributor.authorBagwan, Navratan 
dc.contributor.authorGalan-Arriola, Carlos 
dc.contributor.authorPun-Garcia, Andres 
dc.contributor.authorAguero, Jaume 
dc.contributor.authorFuster, Valentin 
dc.contributor.authorIbanez, Borja 
dc.contributor.authorVazquez, Jesus
dc.identifier.citationSci Rep. 2017; 7(1):12343es_ES
dc.description.abstractReperfusion alters post-myocardial infarction (MI) healing; however, very few systematic studies report the early molecular changes following ischemia/reperfusion (I/R). Alterations in the remote myocardium have also been neglected, disregarding its contribution to post-MI heart failure (HF) development. This study characterizes protein dynamics and contractile abnormalities in the ischemic and remote myocardium during one week after MI. Closed-chest 40 min I/R was performed in 20 pigs sacrificed at 120 min, 24 hours, 4days, and 7days after reperfusion (n = 5 per group). Myocardial contractility was followed up by cardiac magnetic resonance (CMR) and tissue samples were analyzed by multiplexed quantitative proteomics. At early reperfusion (120 min), the ischemic area showed a coordinated upregulation of inflammatory processes, whereas interstitial proteins, angiogenesis and cardio-renal signaling processes increased at later reperfusion (day 4 and 7). Remote myocardium showed decreased contractility at 120 min- and 24 h-CMR accompanied by transient alterations in contractile and mitochondrial proteins. Subsequent recovery of regional contractility was associated with edema formation on CMR and increases in inflammation and wound healing proteins on post-MI day 7. Our results establish for the first time the altered protein signatures in the ischemic and remote myocardium early after I/R and might have implications for new therapeutic targets to improve early post-MI remodeling.es_ES
dc.description.sponsorshipThis study was supported by competitive grants from the Spanish Ministry of Economy and Competitiveness (MINECO) (BIO2015-67580-P) through the Carlos III Institute of Health-Fondo de Investigación Sanitaria (PI13/01979 and PRB2 (IPT13/0001 - ISCIII-SGEFI/FEDER, ProteoRed)), the Fondo Europeo de Desarrollo Regional (FEDER, RD: SAF2013-49663EXP), the Fundación La Marato TV3 and in part by the FP7-PEOPLE-2013-ITN Next generation training in cardiovascular research and innovation-Cardionext. Borja Ibanez and Jesús Vázquez are supported by the Red de Investigación Cardiovascular (RIC) of the Spanish Ministry of Health (RD 12/0042/0054 and RD12/0042/0056). Rodrigo Fernández-Jiménez is a recipient of non-overlapping grants from the Ministry of Economy and Competitiveness through the Instituto de Salud Carlos III (Rio Hortega fellowship); and the Fundació Jesús Serra, the Fundación Interhospitalaria de Investigación Cardiovascular (FIC), and the CNIC (FICNIC Fellowship). Jaume Agüero, Aleksandra Binek, and Navratan Bagwan are FP7-PEOPLE-2013-ITN-Cardionext fellows. This work has been co-funded by FEDER funds. This study forms part of a Master Research Agreement (MRA) between CNIC and Philips Healthcare. The CNIC is supported by the MINECO and the Pro-CNIC Foundation, and is a Severo Ochoa Center of Excellence (MINECO award SEV-2015–0505).es_ES
dc.publisherNature Publishing Group es_ES
dc.titleProteomic footprint of myocardial ischemia/reperfusion injury: Longitudinal study of the at-risk and remote regions in the pig modeles_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.contributor.funderMinisterio de Economía, Industria y Competitividad (España) 
dc.contributor.funderInstituto de Salud Carlos III 
dc.contributor.funderUnión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) 
dc.contributor.funderFundación ProCNIC 
dc.contributor.funderFundacio la Marato
dc.contributor.funderFundación Jesús Serra 
dc.contributor.funderFundación Interhospitalaria de Investigación Cardiovascular 
dc.contributor.funderUnión Europea. Comisión Europea 
dc.contributor.funderPhiliphs Healthcare
dc.contributor.funderCentro de Investigación Biomédica en Red - CIBERCV (Enfermedades Cardiovasculares) 
dc.relation.publisherversionSci Rep. 2017; 7(1):12343es_ES
dc.identifier.journalScientific reportses_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Laboratorio Traslacional para la Imagen y Terapia Cardiovasculares_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Proteómica cardiovasculares_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Imagen Cardiovascular y Estudios Poblacionaleses_ES
dc.repisalud.orgCNICCNIC::Unidades técnicas::Proteómica / Metabolómicaes_ES
dc.rights.accessRightsopen accesses_ES

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