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dc.contributor.author | Martin-Cofreces, Noa B. | |
dc.contributor.author | Robles-Valero, Javier | |
dc.contributor.author | Cabrero, J Román | |
dc.contributor.author | Mittelbrunn, Maria | |
dc.contributor.author | Gordón-Alonso, Mónica | |
dc.contributor.author | Sung, Ching-Hwa | |
dc.contributor.author | Alarcón, Balbino | |
dc.contributor.author | Vázquez, Jesús | |
dc.contributor.author | Sanchez-Madrid, Francisco | |
dc.date.accessioned | 2019-02-05T14:46:31Z | |
dc.date.available | 2019-02-05T14:46:31Z | |
dc.date.issued | 2008-09-08 | |
dc.identifier.citation | J Cell Biol. 2008; 182(5):951-62 | es_ES |
dc.identifier.issn | 0021-9525 | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/7121 | |
dc.description.abstract | The translocation of the microtubule-organizing center (MTOC) toward the nascent immune synapse (IS) is an early step in lymphocyte activation initiated by T cell receptor (TCR) signaling. The molecular mechanisms that control the physical movement of the lymphocyte MTOC remain largely unknown. We have studied the role of the dynein-dynactin complex, a microtubule-based molecular motor, in the process of T cell activation during T cell antigen-presenting cell cognate immune interactions. Impairment of dynein-dynactin complex activity, either by overexpressing the p50-dynamitin component of dynactin to disrupt the complex or by knocking down dynein heavy chain expression to prevent its formation, inhibited MTOC translocation after TCR antigen priming. This resulted in a strong reduction in the phosphorylation of molecules such as zeta chain-associated protein kinase 70 (ZAP70), linker of activated T cells (LAT), and Vav1; prevented the supply of molecules to the IS from intracellular pools, resulting in a disorganized and dysfunctional IS architecture; and impaired interleukin-2 production. Together, these data reveal MTOC translocation as an important mechanism underlying IS formation and sustained T cell signaling. | es_ES |
dc.description.sponsorship | This study was supported by the Instituto de Salud Carlos III, Ministerio de Sanidad y Consumo, Spain (Red Temática de Investigación en Enfermedades Cardiovasculares grant RD06/0021/2006 and postdoctoral fellowship to N.B. Martín-Cófreces), Ministerio de Educación y Ciencia of Spain (grant BFU200508435/BMC and Formación Profesorado Universitario fellowship to J. Robles-Valero), Juan March Foundation (Ayuda a la Investigación Básica 2002) and grant INSINET0159/2006 from Comunidad de Madrid. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Rockefeller University Press | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | * |
dc.subject.mesh | Adaptor Proteins, Signal Transducing | es_ES |
dc.subject.mesh | Antigen-Presenting Cells | es_ES |
dc.subject.mesh | Biological Transport | es_ES |
dc.subject.mesh | CD3 Complex | es_ES |
dc.subject.mesh | Cell Line | es_ES |
dc.subject.mesh | Dynactin Complex | es_ES |
dc.subject.mesh | Dyneins | es_ES |
dc.subject.mesh | Green Fluorescent Proteins | es_ES |
dc.subject.mesh | Humans | es_ES |
dc.subject.mesh | Interleukin-2 | es_ES |
dc.subject.mesh | Jurkat Cells | es_ES |
dc.subject.mesh | Lymphocyte Activation | es_ES |
dc.subject.mesh | Lymphocyte Function-Associated Antigen-1 | es_ES |
dc.subject.mesh | Membrane Proteins | es_ES |
dc.subject.mesh | Microtubule-Associated Proteins | es_ES |
dc.subject.mesh | Microtubule-Organizing Center | es_ES |
dc.subject.mesh | Phosphorylation | es_ES |
dc.subject.mesh | Protein Subunits | es_ES |
dc.subject.mesh | RNA Interference | es_ES |
dc.subject.mesh | Receptors, Antigen, T-Cell | es_ES |
dc.subject.mesh | Signal Transduction | es_ES |
dc.subject.mesh | T-Lymphocytes | es_ES |
dc.title | MTOC translocation modulates IS formation and controls sustained T cell signaling | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Atribución-NoComercial-CompartirIgual 4.0 Internacional | * |
dc.identifier.pubmedID | 18779373 | es_ES |
dc.format.volume | 182 | es_ES |
dc.format.number | 5 | es_ES |
dc.format.page | 951-62 | es_ES |
dc.identifier.doi | 10.1083/jcb.200801014 | es_ES |
dc.contributor.funder | Ministerio de Ciencia, Innovación y Universidades (España) | |
dc.contributor.funder | Ministerio de Sanidad y Consumo (España) | |
dc.contributor.funder | Ministerio de Educación y Ciencia (España) | |
dc.contributor.funder | Comunidad de Madrid (España) | |
dc.contributor.funder | Fundación Juan March | |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 1540-8140 | es_ES |
dc.relation.publisherversion | https://doi.org/10.1083/jcb.200801014 | es_ES |
dc.identifier.journal | The Journal of cell biology | es_ES |
dc.repisalud.orgCNIC | CNIC::Grupos de investigación::Comunicación Intercelular en la Respuesta Inflamatoria | es_ES |
dc.repisalud.institucion | CNIC | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/RD06/0021/2006 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/BFU2005/08435 | es_ES |
dc.rights.accessRights | open access | es_ES |