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dc.contributor.authorMendez, Jairo A
dc.contributor.authorUsme-Ciro, Jose A
dc.contributor.authorDomingo, Cristina 
dc.contributor.authorRey, Gloria J
dc.contributor.authorSanchez, Juan A
dc.contributor.authorTenorio, Antonio 
dc.contributor.authorGallego-Gomez, Juan C
dc.identifier.citationVirol J. 2010 Sep 14;7:226.es_ES
dc.description.abstractBACKGROUND: Dengue Fever is one of the most important viral re-emergent diseases affecting about 50 million people around the world especially in tropical and sub-tropical countries. In Colombia, the virus was first detected in the earliest 70's when the disease became a major public health concern. Since then, all four serotypes of the virus have been reported. Although most of the huge outbreaks reported in this country have involved dengue virus serotype 1 (DENV-1), there are not studies about its origin, genetic diversity and distribution. RESULTS: We used 224 bp corresponding to the carboxyl terminus of envelope (E) gene from 74 Colombian isolates in order to reconstruct phylogenetic relationships and to estimate time divergences. Analyzed DENV-1 Colombian isolates belonged to the formerly defined genotype V. Only one virus isolate was clasified in the genotype I, likely representing a sole introduction that did not spread. The oldest strains were closely related to those detected for the first time in America in 1977 from the Caribbean and were detected for two years until their disappearance about six years later. Around 1987, a split up generated 2 lineages that have been evolving separately, although not major amino acid changes in the analyzed region were found. CONCLUSION: DENV-1 has been circulating since 1978 in Colombia. Yet, the phylogenetic relationships between strains isolated along the covered period of time suggests that viral strains detected in some years, although belonging to the same genotype V, have different recent origins corresponding to multiple re-introduction events of viral strains that were circulating in neighbor countries. Viral strains used in the present study did not form a monophyletic group, which is evidence of a polyphyletic origin. We report the rapid spread patterns and high evolution rate of the different DENV-1 lineages.es_ES
dc.description.sponsorshipThis research was supported by Instituto Colombiano para el Desarrollo de la Ciencia y la Tecnología Francisco José de Caldas - COLCIENCIAS grants 11150416336 CT 234-2004, 11150418079 and 111540820511 from the Colombian government and the Instituto Nacional de Salud resources.es_ES
dc.publisherBiomed Centrales_ES
dc.relation.isversionofPublisher's versiones_ES
dc.subject.meshCluster Analysis es_ES
dc.subject.meshColombia es_ES
dc.subject.meshDengue es_ES
dc.subject.meshDengue Virus es_ES
dc.subject.meshEvolution, Moleculares_ES
dc.subject.meshGenotype es_ES
dc.subject.meshHumans es_ES
dc.subject.meshMolecular Epidemiology es_ES
dc.subject.meshPhylogeny es_ES
dc.subject.meshRNA, Viral es_ES
dc.subject.meshSequence Analysis, DNA es_ES
dc.subject.meshViral Envelope Proteins es_ES
dc.subject.meshPolymorphism, Genetices_ES
dc.titlePhylogenetic history demonstrates two different lineages of dengue type 1 virus in Colombiaes_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.contributor.funderInstituto Colombiano para el Desarrollo de la Ciencia y la Tecnología Francisco José de Caldas (COLCIENCIAS)es_ES
dc.identifier.journalVirology journales_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES

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Atribución 4.0 Internacional
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