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dc.contributor.authorAcebo, Paloma 
dc.contributor.authorMartin-Galiano, Antonio Javier 
dc.contributor.authorNavarro, Sara
dc.contributor.authorZaballos, Angel 
dc.contributor.authorAmblar, Monica 
dc.date.accessioned2019-02-01T15:30:36Z
dc.date.available2019-02-01T15:30:36Z
dc.date.issued2012-03
dc.identifier.citationRNA (2012), 18:530–546.es_ES
dc.identifier.issn1355-8382es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/7066
dc.description.abstractStreptococcus pneumoniae is the main etiological agent of community-acquired pneumonia and a major cause of mortality and morbidity among children and the elderly. Genome sequencing of several pneumococcal strains revealed valuable information about the potential proteins and genetic diversity of this prevalent human pathogen. However, little is known about its transcriptional regulation and its small regulatory noncoding RNAs. In this study, we performed deep sequencing of the S. pneumoniae TIGR4 strain RNome to identify small regulatory RNA candidates expressed in this pathogen. We discovered 1047 potential small RNAs including intragenic, 5'- and/or 3'-overlapping RNAs and 88 small RNAs encoded in intergenic regions. With this approach, we recovered many of the previously identified intergenic small RNAs and identified 68 novel candidates, most of which are conserved in both sequence and genomic context in other S. pneumoniae strains. We confirmed the independent expression of 17 intergenic small RNAs and predicted putative mRNA targets for six of them using bioinformatics tools. Preliminary results suggest that one of these six is a key player in the regulation of competence development. This study is the biggest catalog of small noncoding RNAs reported to date in S. pneumoniae and provides a highly complete view of the small RNA network in this pathogen.es_ES
dc.description.sponsorshipThis work was supported by the Fondo de Investigación Sanitaria (FIS) (PI08/0442 and PI11/00656) and the Ministerio de Ciencia e Innovación (MICINN) (Ramón y Cajal program, RYC-2007-00179). A.J.M. was the recipient of a postdoctoral Miguel Servet contract from the Sistema Nacional de Salud funded by the FIS. During the first part of this work, M.A. was the recipient of a junior researcher contract funded by the Ramon y Cajal program from MICINN. S.N. was the recipient of a grant from CIBER Enfermedades Respiratorias (an initiative of the Instituto de Salud Carlos III).es_ES
dc.language.isoenges_ES
dc.publisherRNA Societyes_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/*
dc.subjectPneumococcuses_ES
dc.subjectSmall noncoding RNAses_ES
dc.subjectTranscriptional regulationes_ES
dc.subjectPathogenesises_ES
dc.subjectCompetencees_ES
dc.subject.meshBase Sequence es_ES
dc.subject.meshComputational Biology es_ES
dc.subject.meshGene Expression es_ES
dc.subject.meshGene Expression Regulation, Bacterial es_ES
dc.subject.meshGenomics es_ES
dc.subject.meshHigh-Throughput Nucleotide Sequencing es_ES
dc.subject.meshHumans es_ES
dc.subject.meshMolecular Sequence Data es_ES
dc.subject.meshNucleic Acid Conformation es_ES
dc.subject.meshRNA, Bacterial es_ES
dc.subject.meshRNA, Small Untranslated es_ES
dc.subject.meshSequence Analysis, RNA es_ES
dc.subject.meshStreptococcus pneumoniae es_ES
dc.titleIdentification of 88 regulatory small RNAs in the TIGR4 strain of the human pathogen Streptococcus pneumoniaees_ES
dc.typeArtículoes_ES
dc.rights.licenseAtribución-NoComercial-4.0 Internacional*
dc.identifier.pubmedID22274957es_ES
dc.format.volume18es_ES
dc.format.number3es_ES
dc.format.page530-46es_ES
dc.identifier.doi10.1261/rna.027359.111es_ES
dc.contributor.funderFondo de Investigaciones Sanitariases_ES
dc.contributor.funderMinisterio de Ciencia e Innovación (España)es_ES
dc.description.peerreviewedes_ES
dc.identifier.e-issn1469-9001es_ES
dc.relation.publisherversionhttps://www.doi.org/10.1261/rna.027359.111es_ES
dc.identifier.journalRNAes_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/PI08/0442es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/PI11/00656es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/RYC-2007-00179es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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Atribución-NoComercial-4.0 Internacional
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