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dc.contributor.authorLucio, Aida de 
dc.contributor.authorAmor Aramendia, Aranzazu 
dc.contributor.authorBailo-Barroso, Begoña 
dc.contributor.authorSaugar, Jose Maria 
dc.contributor.authorAnegagrie, Melaku 
dc.contributor.authorArroyo, Ana
dc.contributor.authorLópez-Quintana, Beatriz
dc.contributor.authorZewdie, Derjew
dc.contributor.authorAyehubizu, Zimmam
dc.contributor.authorYizengaw, Endalew
dc.contributor.authorAbera, Bayeh
dc.contributor.authorYimer, Mulat 
dc.contributor.authorMulu, Wondemagen
dc.contributor.authorHailu, Tadesse 
dc.contributor.authorHerrador, Zaida 
dc.contributor.authorFuentes Corripio, Isabel 
dc.contributor.authorCarmena, David
dc.identifier.citationPLoS One. 2016 Jul 28;11(7):e0159992.es_ES
dc.description.abstractBACKGROUD: Giardia duodenalis and Cryptosporidium spp. are enteric protozoan causing gastrointestinal illness in humans and animals. Giardiasis and cryptosporidiosis are not formally considered as neglected tropical diseases, but belong to the group of poverty-related infectious diseases that impair the development and socio-economic potential of infected individuals in developing countries. METHODS: We report here the prevalence and genetic diversity of G. duodenalis and Cryptosporidium spp. in children attending rural primary schools in the Bahir Dar district of the Amhara Region, Ethiopia. Stool samples were collected from 393 children and analysed by molecular methods. G. duodenalis was detected by real-time PCR, and the assemblages and sub-assemblages were determined by multilocus sequence-based genotyping of the glutamate dehydrogenase and β-giardin genes of the parasite. Detection and identification of Cryptosporidium species was carried out by sequencing of a partial fragment of the small-subunit ribosomal RNA gene. PRINCIPAL FINDINGS: The PCR-based prevalences of G. duodenalis and Cryptosporidium spp. were 55.0% (216/393) and 4.6% (18/393), respectively. A total of 78 G. duodenalis isolates were successfully characterized, revealing the presence of sub-assemblages AII (10.3%), BIII (28.2%), and BIV (32.0%). Discordant typing results AII/AIII and BIII/BIV were identified in 7.7% and 15.4% of the isolates, respectively. An additional five (6.4%) isolates were assigned to assemblage B. No mixed infections of assemblages A+B were found. Extensive genetic variation at the nucleotide level was observed within assemblage B (but no within assemblage A), resulting in the identification of a large number of sub-types. Cryptosporidium diversity was demonstrated by the occurrence of C. hominis, C. parvum, and C. viatorum in the population under study. CONCLUSIONS: Our data suggest an epidemiological scenario with an elevated transmission intensity of a wide range of G. duodenalis genetic variants. Importantly, the elevated degree of genetic diversity observed within assemblage B is consistent with the occurrence of intra-assemblage recombination in G. duodenalis.es_ES
dc.description.sponsorshipThis project was funded by Fundación Mundo Sano (AAA), Argentina Research project CP12/03081 (DC), Carlos III Health Institute, Ministry of Economy and Competitiveness, Spain. It was also supported by Research project PI13/01106 (IF), Carlos III Health Institute, Ministry of Economy and Competitiveness, Spain.es_ES
dc.publisherPublic Library of Science (PLOS) es_ES
dc.subject.meshChild es_ES
dc.subject.meshCryptosporidiosis es_ES
dc.subject.meshCryptosporidium es_ES
dc.subject.meshEthiopia es_ES
dc.subject.meshGiardia lamblia es_ES
dc.subject.meshGiardiasis es_ES
dc.subject.meshHumans es_ES
dc.subject.meshPolymerase Chain Reaction es_ES
dc.subject.meshPrevalence es_ES
dc.subject.meshGenetic Variation es_ES
dc.titlePrevalence and Genetic Diversity of Giardia duodenalis and Cryptosporidium spp. among School Children in a Rural Area of the Amhara Region, North-West Ethiopiaes_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.contributor.funderFundación Mundo Sano 
dc.contributor.funderInstituto de Salud Carlos III 
dc.identifier.journalPloS onees_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.centroISCIII::Centro Nacional de Medicina Tropicales_ES
dc.rights.accessRightsopen accesses_ES

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