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dc.contributor.authorCalvo-Álvarez, Estefanía
dc.contributor.authorÁlvarez-Velilla, Raquel
dc.contributor.authorJimenez, Maribel 
dc.contributor.authorMolina, Ricardo 
dc.contributor.authorPérez-Pertejo, Yolanda
dc.contributor.authorBalaña-Fouce, Rafael
dc.contributor.authorReguera, Rosa M
dc.identifier.citationPLoS Negl Trop Dis. 2014 Sep 4;8(9):e3075es_ES
dc.description.abstractBACKGROUND: The mode of reproduction in Leishmania spp has been argued to be essentially clonal. However, recent data (genetic analysis of populations and co-infections in sand flies) have proposed the existence of a non-obligate sexual cycle in the extracellular stage of the parasite within the sand fly vector. In this article we propose the existence of intraclonal genetic exchange in the natural vector of Leishmania infantum. METHODOLOGY/PRINCIPAL FINDINGS: We have developed transgenic L. infantum lines expressing drug resistance markers linked to green and red fluorescent reporters. We hypothesized whether those cells with identical genotype can recognize each other and mate. Both types of markers were successfully exchanged within the sand fly midgut of the natural vector Phlebotomus perniciosus when individuals from these species were fed with a mixture of parental clones. Using the yellow phenotype and drug resistance markers, we provide evidence for genetic exchange in L. infantum. The hybrid progeny appeared to be triploid based on DNA content analysis. The hybrid clone analyzed was stable throughout the complete parasite life cycle. The progress of infections by the hybrid clone in BALB/c mice caused a reduction in parasite loads in both spleen and liver, and provided weight values similar to those obtained with uninfected mice. Spleen arginase activity was also significantly reduced relative to parental strains. CONCLUSIONS/SIGNIFICANCE: A L. infantum hybrid lineage was obtained from intraclonal genetic exchange within the midgut of the natural vector, suggesting the ability of this parasite to recognize the same genotype and mate. The yellow hybrid progeny is stable throughout the whole parasite life cycle but with a slower virulence, which correlates well with the lower arginase activity detected both in vitro and in vivo infections.es_ES
dc.description.sponsorshipThis study was partially funded by EU grant FP7-2011-261504 EDENext and the paper is catalogued by the EDENext Steering Committee as EDENext. Ministerio de Ciencia y Tecnología (AGL2010-16078/GAN), CYTED 214RT0482 and Instituto de Salud Carlos III (PI12/00104) partially supported this research. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.es_ES
dc.publisherPublic Library of Sciencees_ES
dc.relation.isversionofPublisher's versiones_ES
dc.subject.meshAnimals es_ES
dc.subject.meshDigestive System es_ES
dc.subject.meshFemale es_ES
dc.subject.meshFlow Cytometry es_ES
dc.subject.meshHumans es_ES
dc.subject.meshHybridization, Genetic es_ES
dc.subject.meshLeishmania infantum es_ES
dc.subject.meshLeishmaniasis, Visceral es_ES
dc.subject.meshLife Cycle Stages es_ES
dc.subject.meshMice es_ES
dc.subject.meshMice, Inbred BALB C es_ES
dc.subject.meshPhlebotomus es_ES
dc.titleFirst evidence of intraclonal genetic exchange in trypanosomatids using two Leishmania infantum fluorescent transgenic cloneses_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.contributor.funder7º Programa Marco-Comisión Europeaes_ES
dc.contributor.funderMinisterio de Ciencia y Tecnología (España)es_ES
dc.contributor.funderPrograma Iberoamericano de Ciencia y Tecnología para el Desarrollo (CYTED)es_ES
dc.contributor.funderInstituto de Salud Carlos III-ISCIIIes_ES
dc.identifier.journalPLoS neglected tropical diseaseses_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES

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Atribución 4.0 Internacional
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