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dc.contributor.authorCleton, Natalie B
dc.contributor.authorGodeke, Gert-Jan
dc.contributor.authorReimerink, Johan
dc.contributor.authorBeersma, Mathias F
dc.contributor.authorDoorn, H Rogier van
dc.contributor.authorFranco, Leticia 
dc.contributor.authorGoeijenbier, Marco
dc.contributor.authorJimenez-Clavero, Miguel A
dc.contributor.authorJohnson, Barbara W
dc.contributor.authorNiedrig, Matthias
dc.contributor.authorPapa, Anna
dc.contributor.authorSambri, Vittorio
dc.contributor.authorTami, Adriana
dc.contributor.authorVelasco-Salas, Zoraida I
dc.contributor.authorKoopmans, Marion P G
dc.contributor.authorReusken, Chantal B E M
dc.date.accessioned2018-12-19T12:18:47Z
dc.date.available2018-12-19T12:18:47Z
dc.date.issued2015-03-13
dc.identifier.citationPLoS Negl Trop Dis. 2015 Mar 13;9(3):e0003580es_ES
dc.identifier.issn1935-2735es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/6909
dc.description.abstractBACKGROUND: The family Flaviviridae, genus Flavivirus, holds many of the world's most prevalent arboviral diseases that are also considered the most important travel related arboviral infections. In most cases, flavivirus diagnosis in travelers is primarily based on serology as viremia is often low and typically has already been reduced to undetectable levels when symptoms set in and patients seek medical attention. Serological differentiation between flaviviruses and the false-positive results caused by vaccination and cross-reactivity among the different species, are problematic for surveillance and diagnostics of flaviviruses. Their partially overlapping geographic distribution and symptoms, combined with increase in travel, and preexisting antibodies due to flavivirus vaccinations, expand the need for rapid and reliable multiplex diagnostic tests to supplement currently used methods. GOAL: We describe the development of a multiplex serological protein microarray using recombinant NS1 proteins for detection of medically important viruses within the genus Flavivirus. Sera from clinical flavivirus patients were used for primary development of the protein microarray. RESULTS: Results show a high IgG and IgM sensitivity and specificity for individual NS1 antigens, and limited cross reactivity, even within serocomplexes. In addition, the serology based on this array allows for discrimination between infection and vaccination response for JEV vaccine, and no cross-reactivity with TBEV and YFV vaccine induced antibodies when testing for antibodies to other flaviviruses. CONCLUSION: Based on these data, multiplex NS1-based protein microarray is a promising tool for surveillance and diagnosis of flaviviruses.es_ES
dc.description.sponsorshipThe authors received no specific funding for this work.es_ES
dc.language.isoenges_ES
dc.publisherPublic Library of Science (PLOS) es_ES
dc.type.hasVersionVoRes_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.meshAntibodies, Viral es_ES
dc.subject.meshCross Reactions es_ES
dc.subject.meshEpitopes es_ES
dc.subject.meshFemale es_ES
dc.subject.meshFlavivirus Infections es_ES
dc.subject.meshHumans es_ES
dc.subject.meshMale es_ES
dc.subject.meshMiddle Aged es_ES
dc.subject.meshProtein Array Analysis es_ES
dc.subject.meshViral Nonstructural Proteins es_ES
dc.subject.meshTravel es_ES
dc.titleSpot the difference-development of a syndrome based protein microarray for specific serological detection of multiple flavivirus infections in travelerses_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID25767876es_ES
dc.format.volume9es_ES
dc.format.number3es_ES
dc.format.pagee0003580es_ES
dc.identifier.doi10.1371/journal.pntd.0003580es_ES
dc.description.peerreviewedes_ES
dc.identifier.e-issn1935-2735es_ES
dc.relation.publisherversionhttps://doi.org/10.1371/journal.pntd.0003580es_ES
dc.identifier.journalPLoS neglected tropical diseaseses_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.rights.accessRightsopen accesses_ES


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Atribución 4.0 Internacional
Este Item está sujeto a una licencia Creative Commons: Atribución 4.0 Internacional