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dc.contributor.authorMagnet, Angela
dc.contributor.authorGomes, Thiago Santos
dc.contributor.authorPardinas, Carmen
dc.contributor.authorGarcia de Blas, Natalia
dc.contributor.authorSadaba, Cruz
dc.contributor.authorCarrillo, Eugenia 
dc.contributor.authorIzquierdo, Fernando
dc.contributor.authorDel Castillo, José Manuel Benítez
dc.contributor.authorDel Aguila, Carmen
dc.contributor.authorFenoy, Soledad
dc.contributor.authorHurtado, Carolina
dc.identifier.citationParasit Vectors. 2018 Jan 22;11(1):50es_ES
dc.description.abstractBACKGROUND: The use of contact lenses has increased in recent years as has the incidence of Dry Eye Syndrome, partly due to their use. Artificial tears are the most common treatment option. Since these changes can facilitate Acanthamoeba infection, the present study has been designed to evaluate the effect of three artificial tears treatments in the viability of Acanthamoeba genotype T4 trophozoites. Optava Fusion™, Oculotect®, and Artelac® Splash were selected due to their formulation. METHODS: Viability was assessed using two staining methods, Trypan Blue stain and CTC stain at different time intervals (2, 4, 6, 8 and 24 h). Trypan Blue viability was obtained by manual count with light microscopy while the CTC stain was determined using flow cytometry. RESULTS: Trypan Blue staining results demonstrated a decrease in viability for Optava Fusion™ and Artelac® Splash during the first 4 h of incubation. After, this effect seems to lose strength. In the case of Oculotect®, complete cell death was observed after 2 h. Using flow cytometry analysis, Optava Fusion™ and Oculotect® exhibited the same effect observed with Trypan Blue staining. However, Artelac® Splash revealed decreasing cell respiratory activity after four hours, with no damage to the cell membrane. CONCLUSIONS: The present study uses, for the first time, CTC stain analyzed by flow cytometry to establish Acanthamoeba viability demonstrating its usefulness and complementarity with the traditional stain, Trypan Blue. Artelac® Splash, with no preservatives, and Optava Fusion TM, with Purite®, have not shown any useful amoebicidal activity. On the contrary, promising results presented by Ocultect®, with BAK, open up a new possibility for Acanthamoeba keratitis prophylaxis and treatment although in vivo studies should be carried out.es_ES
dc.description.sponsorshipThis project was supported by Grant PI12/ 02725 from Instituto de Salud Carlos III (FISS) and FEDER by grant USPCEU-PC07/2013 of the Fundación Universitaria San Pablo CEU and by grant CEU-Banco de Santander. TSG was supported by Research Program EADS Construcciones Aeronáuticas S.A. - Brasil I and by CAPES/Brazil (Grant BEX 9132/13-9).es_ES
dc.publisherBioMed Central (BMC) es_ES
dc.subjectAmoebicidal effectes_ES
dc.subjectArtificial tearses_ES
dc.titleCan artificial tears prevent Acanthamoeba keratitis? An in vitro approaches_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.contributor.funderInstituto de Salud Carlos III 
dc.contributor.funderUnión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) 
dc.contributor.funderFundación Universitaria San Pablo CEU 
dc.contributor.funderBanco Santander 
dc.identifier.journalParasites & vectorses_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.rights.accessRightsopen accesses_ES

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