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dc.contributor.authorNieto, Concha
dc.contributor.authorSadowy, Ewa
dc.contributor.authorde la Campa, Adela G 
dc.contributor.authorHryniewicz, Waleria
dc.contributor.authorEspinosa, Manuel
dc.identifier.citationPLoS One. 2010 Jun 23;5(6):e11289.es_ES
dc.description.abstractType II (proteic) chromosomal toxin-antitoxin systems (TAS) are widespread in Bacteria and Archaea but their precise function is known only for a limited number of them. Out of the many TAS described, the relBE family is one of the most abundant, being present in the three first sequenced strains of Streptococcus pneumoniae (D39, TIGR4 and R6). To address the function of the pneumococcal relBE2Spn TAS in the bacterial physiology, we have compared the response of the R6-relBE2Spn wild type strain with that of an isogenic derivative, Delta relB2Spn under different stress conditions such as carbon and amino acid starvation and antibiotic exposure. Differences on viability between the wild type and mutant strains were found only when treatment directly impaired protein synthesis. As a criterion for the permanence of this locus in a variety of clinical strains, we checked whether the relBE2Spn locus was conserved in around 100 pneumococcal strains, including clinical isolates and strains with known genomes. All strains, although having various types of polymorphisms at the vicinity of the TA region, contained a functional relBE2Spn locus and the type of its structure correlated with the multilocus sequence type. Functionality of this TAS was maintained even in cases where severe rearrangements around the relBE2Spn region were found. We conclude that even though the relBE2Spn TAS is not essential for pneumococcus, it may provide additional advantages to the bacteria for colonization and/or infection.es_ES
dc.description.sponsorshipThe research was performed under a collaborative project financed by the European Union (EU-CP223111, CAREPNEUMO, to M.E., E.S. and W.H.), by the Comunidad de Madrid (CM-BIO0260-2006, COMBACT, to M.E. and A.G.C.), and by the Spanish Ministry of Science and Innovation (Grants BIO2008-02154 to A.G.C, and BFU2007-63575 and CSD-2008-00013, INTERMODS, to M.E.). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.es_ES
dc.publisherPublic Library of Sciencees_ES
dc.relation.isversionofPublisher's versiones_ES
dc.subject.meshAnti-Bacterial Agents es_ES
dc.subject.meshAntitoxins es_ES
dc.subject.meshBacterial Toxins es_ES
dc.subject.meshMutation es_ES
dc.subject.meshOperon es_ES
dc.subject.meshStreptococcus pneumoniae es_ES
dc.titleThe relBE2Spn toxin-antitoxin system of Streptococcus pneumoniae: role in antibiotic tolerance and functional conservation in clinical isolateses_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.contributor.funderEuropean Union
dc.contributor.funderGobierno de la Comunidad Autónoma de Madrid
dc.contributor.funderMinisterio de Ciencia e Innovación (España)
dc.identifier.journalPloS onees_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES

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