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dc.contributor.authorGuerra-Neira, Ana 
dc.contributor.authorRubio Muñoz, Jose Miguel 
dc.contributor.authorRoche, Jesus 
dc.contributor.authorOchando, Jordi 
dc.contributor.authorSarrión Auñón, Antonio
dc.contributor.authorBerzosa, Pedro 
dc.contributor.authorBenito, Agustin
dc.identifier.citationInt J Health Geogr. 2006; 5: 27.es_ES
dc.description.abstractBACKGROUND: In this paper we analyse the Plasmodium sp. prevalence in three villages with different isolation status on the island of Bioko (Equatorial Guinea) where malaria is a hyper-endemic disease. We also describe the genetic diversity of P. falciparum, using several plasmodia proteins as markers which show a high degree of polymorphism (MSP-1 and MSP-2). The results obtained from three different populations are compared in order to establish the impact of human movements and interventions. METHODS: Plasmodium sp. were analysed in three villages on Bioko Island (Equatorial Guinea), one of which (Southern) is isolated by geographical barriers. The semi-nested multiplex polymerase chain reaction (PCR) technique was used to determine the prevalence of the four human plasmodia species. The genotyping and frequency of P. falciparum populations were determined by PCR assay target polymorphism regions of the merozoite surface proteins 1 and 2 genes (MSP-1 and MSP-2). RESULTS: The data obtained show that there are no differences in plasmodia population flow between the Northwest and Eastern regions as regards the prevalence of the different Plasmodium species. The Southern population, on the other hand, shows a minor presence of P. malariae and a higher prevalence of P. ovale, suggesting some kind of transmission isolated from the other two. The P. falciparum genotyping in the different regions points to a considerable allelic diversity in the parasite population on Bioko Island, although this is somewhat higher in the Southern region than the others. There was a correlation between parasitaemia levels and the age of the individual with the multiplicity of infection (MOI). CONCLUSION: Results could be explained by the selection of particular MSP alleles. This would tend to limit diversity in the parasite population and leading up to the extinction of rare alleles. On the other hand, the parasite population in the isolated village has less outside influence and the diversity of P. falciparum is maintained higher. The knowledge of parasite populations and their relationships is necessary to study their implications for control intervention.es_ES
dc.description.sponsorshipThis Study was supported by the Fondo de Investigación Sanitaria from Spain (FIS 96/0216) and the Spanish Agency of International Cooperation. J.M. Rubio holds a post-doctoral fellowship from the Comunidad Autonoma de Madrid. Thanks to Malaria National Control Programme of Equatorial Guinea.es_ES
dc.publisherBioMed Central (BMC) 
dc.subject.meshAlleles es_ES
dc.subject.meshAnimals es_ES
dc.subject.meshChild es_ES
dc.subject.meshChild, Preschool es_ES
dc.subject.meshEquatorial Guinea es_ES
dc.subject.meshGenetic Variation es_ES
dc.subject.meshHumans es_ES
dc.subject.meshParasitemia es_ES
dc.subject.meshPlasmodium falciparum es_ES
dc.subject.meshPolymerase Chain Reaction es_ES
dc.subject.meshPrevalence es_ES
dc.subject.meshSpecies Specificity es_ES
dc.titlePlasmodium diversity in non-malaria individuals from the Bioko Island in Equatorial Guinea (West Central-Africa)es_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 2.0 Internacional*
dc.contributor.funderInstituto de Salud Carlos III 
dc.contributor.funderAgencia Española de Cooperación Internacional para el Desarrollo 
dc.identifier.journalInternational journal of health geographicses_ES
dc.repisalud.centroISCIII::Centro Nacional de Medicina Tropicales_ES
dc.rights.accessRightsopen accesses_ES

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