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dc.contributor.authorMoreno, Marta 
dc.contributor.authorOchando, Jordi 
dc.contributor.authorNzambo, Sisinio
dc.contributor.authorBobuakasi, Leonardo
dc.contributor.authorBuatiche, Jesús N
dc.contributor.authorOndo, Melchor
dc.contributor.authorMicha, Francisco
dc.contributor.authorBenito, Agustin
dc.identifier.citationMalar J. 2004 Jul 6:3:20.es_ES
dc.description.abstractBACKGROUND: A study was carried out in a village of the mainland region of Equatorial Guinea in order to ascertain a) which members of Anopheles gambiae complex could be involved in malaria transmission and b) the rate of infectivity for Anopheles melas comparing two different methods, a PCR able to detect sporozoite-DNA and an immunochromatographic assay MPR (Malaria Rapid Dipstick Panel Assay). METHODS: Mosquitoes were sampled at night by indoor captures in two houses of a coastal village in Equatorial Guinea (Ayantang). Collected mosquitoes were identified as An. gambiae s.l. These were individually dried into silica-gel. The head-thorax of the An. gambiae s.l. mosquitoes were analysed by PCR to verify that the species was of the gambiae complex. Individual head-thorax and pools (5 pools) of homogenized mosquitoes employed in Malaria Rapid Panel assay (MRP assay) were lysed and DNA was extracted. PCR was designed from the 753 base pair insert of pBRKl-14 and DNA was amplified. The relationship between dipstick and PCR to detect Plasmodium falciparum sporozoites was measured in terms of sensitivity, specificity and test association (Cohen's kappa value). RESULTS: Two hundred and sixty-four An. gambiae s.l. females were studied (214 individually and five pools with 10 mosquitoes in each). PCR analysis showed that 207 mosquitoes were An. melas, 3 An. gambiae s.s. and 4 could not be identified. By using PCR as the gold standard method when dipstick assay was compared, matching results were obtained for 6 mosquitoes and, in one case MRP was positive while PCR was not reactive. MRP assay showed a low sensitivity (3.3%) when compared with falciparum-DNA detection (17,7% and 14,3%, series A and B respectively). Agreement between the two test formats was low (kappa = 0,224). CONCLUSION: It was determined that An. melas is the main anopheline vector involved in malaria transmission in Ayantang, a coastal village in mainland Equatorial Guinea. A comparison of PCR and Vec-Test Assay, concluded that the PCR method proved to be a more sensitive and useful tool than the dipstick assay to determine the malarial infection rate in mosquitoes in an area of stable and high malaria transmission like Equatorial Guinea.es_ES
dc.description.sponsorshipThis work was supported by the Spanish Agency of International Cooperation (AECI), the Institute of Health Carlos III and by the Research Network of Centres for Tropical Medicine (RICET), supported by FIS. Nick Service made comments on the manuscript and helped with the language revision.es_ES
dc.publisherBioMed Central (BMC) 
dc.subject.meshAnimals es_ES
dc.subject.meshAnopheles es_ES
dc.subject.meshDNA, Protozoan es_ES
dc.subject.meshEnzyme-Linked Immunosorbent Assay es_ES
dc.subject.meshEquatorial Guinea es_ES
dc.subject.meshFemale es_ES
dc.subject.meshHumans es_ES
dc.subject.meshInsect Vectors es_ES
dc.subject.meshMalaria, Falciparum es_ES
dc.subject.meshPlasmodium falciparum es_ES
dc.subject.meshPolymerase Chain Reaction es_ES
dc.subject.meshSensitivity and Specificity es_ES
dc.titleMalaria Panel Assay versus PCR: detection of naturally infected Anopheles melas in a coastal village of Equatorial Guineaes_ES
dc.typeresearch articlees_ES
dc.rights.licenseAtribución 2.0*
dc.contributor.funderAgencia Española de Cooperación Internacional para el Desarrollo 
dc.contributor.funderInstituto de Salud Carlos III 
dc.contributor.funderRETICS-Investigación colaborativa en Enfermedades Tropicales (RICET-ISCIII) (España) 
dc.identifier.journalMalaria journales_ES
dc.repisalud.centroISCIII::Centro Nacional de Medicina Tropicales_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.rights.accessRightsopen accesses_ES

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