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dc.contributor.authorMartinez-Pinna, Roxana
dc.contributor.authorBurillo, Elena
dc.contributor.authorMadrigal-Matute, Julio
dc.contributor.authorLopez, Juan Antonio 
dc.contributor.authorCamafeita, Emilio 
dc.contributor.authorTorres-Fonseca, Monica Maria
dc.contributor.authorLlamas-Granda, Patricia
dc.contributor.authorEgido, Jesus
dc.contributor.authorMichel, Jean-Baptiste
dc.contributor.authorBlanco-Colio, Luis Miguel
dc.contributor.authorMartin-Ventura, Jose Luis
dc.date.accessioned2018-11-29T15:05:12Z
dc.date.available2018-11-29T15:05:12Z
dc.date.issued2014-08
dc.identifier.citationProteomics Clin Appl. 2014 Aug;8(7-8):626-30es_ES
dc.identifier.issn1862-8346es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/6744
dc.description.abstractPURPOSE: To test whether red blood cell (RBC) membrane composition is modified in abdominal aortic aneurysms (AAA) patients. EXPERIMENTAL DESIGN: RBC membrane extracts from AAA patients (aortic diameter >3 cm, n = 7) and control subjects (n = 4) were analyzed by a label-free quantitative MS-based strategy, using spectral count data. Additional validation was performed by western-blot. RESULTS: Data analysis based on spectral count from MS/MS-based experiments provided us a signature of 39 proteins differentially expressed in RBC membranes between AAA and controls (changes equal/over 1.515-fold; p-values equal/lower 0.05). MS data revealed altered levels of structural membrane proteins (e.g. spectrins and ankyrin), components of the degradation machinery (proteasome subunits), and oxidative stress related proteins (e.g. catalase and peroxiredoxin-2) among others. Decreased catalase and peroxiredoxin-2 expression in RBC membrane of AAA patients compared to controls were further validated by Western blot, confirming the proteomic results. CONCLUSIONS AND CLINICAL RELEVANCE: RBCs membrane protein composition is altered in AAA patients, which could be involved in the pathological role of RBCs in aortic tissue and become potential targets to prevent AAA progression.es_ES
dc.language.isoenges_ES
dc.relation.isversionofPreprintes_ES
dc.relation.isversionofhttps://doi.org/10.1002/prca.201400035
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectAbdominal aortic aneurysmes_ES
dc.subjectRed blood cellses_ES
dc.subject.meshAortic Aneurysm, Abdominal es_ES
dc.subject.meshCase-Control Studies es_ES
dc.subject.meshErythrocyte Membrane es_ES
dc.subject.meshHumans es_ES
dc.subject.meshProteomics es_ES
dc.subject.meshTranscriptome es_ES
dc.titleLabel-free proteomic analysis of red blood cell membrane fractions from abdominal aortic aneurysm patientses_ES
dc.typeArtículoes_ES
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.identifier.pubmedID24976601es_ES
dc.format.volume8es_ES
dc.format.number7-8es_ES
dc.format.page626-30es_ES
dc.identifier.doi10.1002/prca.201400035es_ES
dc.contributor.funderMinisterio de Ciencia e Innovación (España)
dc.contributor.funderMinisterio de Sanidad y Consumo (España)
dc.contributor.funderInstituto de Salud Carlos III - ISCIII
dc.description.peerreviewedes_ES
dc.identifier.journalProteomics. Clinical applicationses_ES
dc.repisalud.orgCNICCNIC::Unidades técnicas::Proteómica / Metabolómicaes_ES
dc.repisalud.institucionCNICes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2013-42525es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/RD12/0042/00038es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/RD09/0076/00101es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
This item is licensed under a: Attribution-NonCommercial-NoDerivatives 4.0 Internacional