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dc.contributor.authorvan Dinther, Dieke
dc.contributor.authorVeninga, Henrike
dc.contributor.authorIborra, Salvador 
dc.contributor.authorBorg, Ellen G. F.
dc.contributor.authorHoogterp, Leoni
dc.contributor.authorOlesek, Katarzyna
dc.contributor.authorBeijer, Marieke R.
dc.contributor.authorSchetters, Sjoerd T. T.
dc.contributor.authorKalay, Hakan
dc.contributor.authorGarcia-Vallejo, Juan J.
dc.contributor.authorFranken, Kees L.
dc.contributor.authorCham, Lamin B.
dc.contributor.authorLang, Karl S.
dc.contributor.authorvan Kooyk, Yvette
dc.contributor.authorSancho, David 
dc.contributor.authorCrocker, Paul R.
dc.contributor.authorden Haan, Joke M. M.
dc.identifier.citationCell Rep. 2018; 22(6):1484-1495
dc.description.abstractSplenic CD169(+) macrophages are located in the marginal zone to efficiently capture blood-borne pathogens. Here, we investigate the requirements for the induction of CD8(+) T cell responses by antigens (Ags) bound by CD169(+) macrophages. Upon Ag targeting to CD169(+) macrophages, we show that BATF3-dependent CD8 alpha(+) dendritic cells (DCs) are crucial for DNGR-1-mediated cross-priming of CD8(+) T cell responses. In addition, we demonstrate that CD169, a sialic acid binding lectin involved in cell-cell contact, preferentially binds to CD8 alpha(+) DCs and that Ag transfer to CD8 alpha(+) DCs and subsequent T cell activation is dependent on the sialic acid-binding capacity of CD169. Finally, functional CD169 mediates optimal CD8(+) T cell responses to modified vaccinia Ankara virus infection. Together, these data indicate that the collaboration of CD169(+) macrophages and CD8 alpha(+) DCs for the initiation of effective CD8(+) T cell responses is facilitated by binding of CD169 to sialic acid containing ligands on CD8 alpha(+) DCs.
dc.description.sponsorshipWe thank J.J. Koning for help on IMARIS and J.P. Middelberg, C. Prins, and R. van der Laan for animal care. We acknowledge the NIH Tetramer Core Facility (contract HHSN272201300006C) for MHC-I tetramers. We thank Mariano Esteban for the MVA-GFP strain. Work in the D.S. laboratory is funded by the CNIC; grant SAF2016-79040-R from the Spanish Ministry of Economy, Industry and Competitiveness (MINECO), Agencia Estatal de Investigacion and FEDER (European fund for Regional Development); Foundation Acteria; and the European Commission (635122-PROCROP H2020) and the European Research Council (ERC-2016-Consolidator Grant 725091). This work was supported by grants from the Dutch Cancer Society (VU2009-4504 and VU2013-5940) and by VUmc CCA (2015-5-22 to J.M.M.d.H.). S.I. is funded by grant SAF2015-74561-JIN.
dc.publisherCell Press
dc.relation.isversionofPublisher's version
dc.titleFunctional CD169 on Macrophages Mediates Interaction with Dendritic Cells for CD8(+) T Cell Cross-Priming
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.contributor.funderNational Institutes of Health
dc.contributor.funderCentro Nacional de Investigaciones Cardiovasculares
dc.contributor.funderMinisterio de Economía, Industria y Competitividad (España)
dc.contributor.funderAgencia Estatal de Investigacion (España)
dc.contributor.funderEuropean Regional Development Fund (ERDF/FEDER)
dc.contributor.funderFondation Acteria
dc.contributor.funderEuropean Commission
dc.contributor.funderEuropean Research Council
dc.contributor.funderDutch Cancer Society
dc.identifier.journalCell Reports
dc.repisalud.orgCNICCNIC::Grupos de investigación::Inmunobiología

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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
This item is licensed under a: Attribution-NonCommercial-NoDerivatives 4.0 Internacional