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dc.contributor.authorBlazquez, Rebeca
dc.contributor.authorSánchez-Margallo, Francisco Miguel
dc.contributor.authorAlvarez, Veronica
dc.contributor.authorMatilla, Elvira
dc.contributor.authorHernandez, Nuria
dc.contributor.authorMarinaro, Federica
dc.contributor.authorGomez-Serrano, Maria 
dc.contributor.authorJorge, Inmaculada 
dc.contributor.authorCasado, Javier G.
dc.contributor.authorMacias-Garcia, Beatriz
dc.date.accessioned2018-11-22T08:10:51Z
dc.date.available2018-11-22T08:10:51Z
dc.date.issued2018
dc.identifierISI:000430660600036
dc.identifier.citationPLoS One. 2018; 13(4):e0196080
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/20.500.12105/6675
dc.description.abstractEndometrial Mesenchymal Stromal Cells (endMSCs) are multipotent cells with immunomodulatory and pro-regenerative activity which is mainly mediated by a paracrine effect. The exosomes released by MSCs have become a promising therapeutic tool for the treatment of immune-mediated diseases. More specifically, extracellular vesicles derived from endMSCs (EV-endMSCs) have demonstrated a cardioprotective effect through the release of anti-apoptotic and pro-angiogenic factors. Here we hypothesize that EV-endMSCs may be used as a co-adjuvant to improve in vitro fertilization outcomes and embryo quality. Firstly, endMSCs and EV-endMSCs were isolated and phenotypically characterized for in vitro assays. Then, in vitro studies were performed on murine embryos co-cultured with EV-endMSCs at different concentrations. Our results firstly demonstrated a significant increase on the total blastomere count of expanded murine blastocysts. Moreover, EV-endMSCs triggered the release of pro-angiogenic molecules from embryos demonstrating an EV-endMSCs concentration-dependent increase of VEGF and PDGF-AA. The release of VEGF and PDGF-AA by the embryos may indicate that the beneficial effect of EV-endMSCs could be mediating not only an increase in the blastocyst's total cell number, but also may promote endometrial angiogenesis, vascularization, differentiation and tissue remodeling. In summary, these results could be relevant for assisted reproduction being the first report describing the beneficial effect of human EV-endMSCs on embryo development.
dc.description.sponsorshipThis work was supported in part by CIBER-CV (CB16/11/00494). One grant from Junta de Extremadura (Ayuda a grupos catalogados de la Junta de Extremadura, GR15175). Two grants from Junta de Extremadura to JGC (TA13042 and 1616168 co-financed by FEDER/FSE). One grant to B-MC (IB16159 co-financed by FEDER/FSE). One grant ``Miguel Servet I´´ from Instituto de Salud Carlos III to JGC (CP17/00021 co-financed by FEDER/FSE). One grant ``Juan de la Cierva´´ to B-MC from Spanish Ministry of Economy, Industry and Competitiveness (IJCI-2014-19428). The funders had no role in study designs, data collection and analysis, decision to publish or preparation of the manuscript.
dc.language.isoeng
dc.publisherPublic Library of Science
dc.relation.isversionofPublisher's version
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectENDOTHELIAL GROWTH-FACTOR
dc.subjectMESENCHYMAL STEM-CELLS
dc.subjectIN-VITRO
dc.subjectCULTURE-MEDIUM
dc.subjectMOUSE EMBRYO
dc.subjectAVIAN EMBRYO
dc.subjectIMPLANTATION
dc.subjectEXOSOMES
dc.subjectANGIOGENESIS
dc.subjectPDGF
dc.titleMurine embryos exposed to human endometrial MSCs-derived extracellular vesicles exhibit higher VEGF/PDGF AA release, increased blastomere count and hatching rates
dc.typeArtículo
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID29684038
dc.format.volume13
dc.identifier.doi10.1371/journal.pone.0196080
dc.contributor.funderCentro de Investigación Biomedica en Red - CIBER
dc.contributor.funderJunta de Extremadura
dc.contributor.funderEuropean Regional Development Fund (ERDF/FEDER)
dc.contributor.funderInstituto de Salud Carlos III - ISCIII
dc.contributor.funderMinisterio de Economía, Industria y Competitividad (España)
dc.description.peerreviewed
dc.relation.publisherversionhttps://doi.org/10.1371/journal.pone.0196080
dc.identifier.journalPLoS ONE
dc.repisalud.orgCNICCNIC::Grupos de investigación::Proteómica cardiovascular
dc.repisalud.institucionCNIC
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/CB16/11/00494es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/CP17/00021es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/IJCI-2014-19428es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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