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dc.contributor.authorIbarra-Meneses, Ana Victoria 
dc.contributor.authorCruz, Israel 
dc.contributor.authorChicharro, Carmen 
dc.contributor.authorSánchez, Carmen 
dc.contributor.authorBiéler, Sylvain
dc.contributor.authorBroger, Tobias
dc.contributor.authorMoreno, Javier 
dc.contributor.authorCarrillo, Eugenia
dc.identifier.citationParasit Vectors. 2018 Apr 17;11(1):250.es_ES
dc.description.abstractBACKGROUND: Nucleic acid amplification tests (NAATs) have proven to be advantageous in the diagnosis of leishmaniases, allowing sensitive diagnosis of: (i) cutaneous leishmaniasis in long duration lesions and (ii) visceral leishmaniasis using a less-invasive sample like peripheral blood, in opposition to tissue aspiration required for parasite demonstration by microscopy. Despite their benefits, the implementation of NAATs for leishmaniasis diagnosis at the point-of-care has not been achieved yet, mostly due to the complexity and logistical issues associated with PCR-based methods. METHODS: In this work, we have evaluated the performance of a ready-to-use loop-mediated isothermal amplification (LAMP) kit using two real time fluorimeters to amplify leishmanial DNA obtained by silica column-based and Boil & Spin protocols. RESULTS: The different approaches used to run and interpret the LAMP reactions showed a performance equivalent to PCR and real-time PCR, using spiked and clinical samples. The time to positivity obtained with real-time fluorimetry showed an excellent correlation with both Ct values and parasite load from real-time quantitative PCR. CONCLUSIONS: The results obtained open the possibility of using a highly stable, ready-to-use LAMP kit for the accurate diagnosis of leishmaniasis at the point-of-care. Furthermore, the feasibility of relating time to positivity, determined with a portable real-time fluorimeter, with the parasite burden could have a wider application in the management of leishmaniasis, such as in treatment efficacy monitoring or as a pharmacodynamics tool in clinical trials.es_ES
dc.description.sponsorshipThis work was supported by funds from the Federal Ministry of Education and Research, Germany (KfW grant reference number 202060457, Development of Products for the Prevention, Diagnosis and Treatment of Neglected and Poverty Related Diseases; and the Ministry of Foreign Affairs, Government of the Netherlands (Activity Ref. Nr. 22211, Developing Innovative Diagnostics to Address Poverty-Related Diseases; E Carrillo was supported by a contract from RD16CIII/0003/0002 Red de Investigación Cooperativa de Enfermedades Tropicales, Subprograma RETICS del Plan Estatal de I+D+I 2013–2016, co-funded by FEDER “Una manera de hacer Europa” funds. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.es_ES
dc.publisherBioMed Central Ltdes_ES
dc.relation.isversionofPublisher's versiones_ES
dc.subjectBoil & Spines_ES
dc.subjectLess-invasive diagnosises_ES
dc.subjectLoop-mediated isothermal amplificationes_ES
dc.subjectLoopamp™ Leishmania detection kites_ES
dc.subjectNon-invasive diagnosises_ES
dc.subjectReal-time fluorimeterses_ES
dc.subjectVisceral leishmaniasises_ES
dc.subject.meshColorimetry es_ES
dc.subject.meshFluorometry es_ES
dc.subject.meshHumans es_ES
dc.subject.meshLeishmania es_ES
dc.subject.meshLeishmaniasis es_ES
dc.subject.meshMolecular Diagnostic Techniques es_ES
dc.subject.meshNucleic Acid Amplification Techniques es_ES
dc.subject.meshPoint-of-Care Testing es_ES
dc.titleEvaluation of fluorimetry and direct visualization to interpret results of a loop-mediated isothermal amplification kit to detect Leishmania DNAes_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.contributor.funderFederal Ministry of Education and Research (Germany)es_ES
dc.contributor.funderMinistry of Foreign Affairs (The Netherlands)es_ES
dc.identifier.journalParasites & vectorses_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES

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Atribución 4.0 Internacional
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