Mostrar el registro sencillo del ítem

dc.contributor.authorGonzalez, Luis Miguel 
dc.contributor.authorCuevas, Laureano 
dc.contributor.authorPerez-Pastrana, Esperanza 
dc.contributor.authorSanta-María, Ysmael
dc.contributor.authorBenito, Agustin 
dc.date.accessioned2018-11-20T09:49:13Z
dc.date.available2018-11-20T09:49:13Z
dc.date.issued2009-10-09
dc.identifier.citationMol Biol Rep. 2010;37(6):2893-902.es_ES
dc.identifier.issn0301-4851es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/6634
dc.description.abstractTo identity immunodiagnostic antigen genes, a Plasmodium falciparum (Dd2 clone) expression library was screened using human immune sera. The ring-infected erythrocyte surface antigen (RESA) was isolated: this antigen of the resistant clone presents repeat tandem sequences like the 3D7 clone, albeit in different numbers. RESA has been studied as a marker of genetic diversity, with different sizes being observed in different isolates and clones of Plasmodium falciparum. The native protein was localised in cultures by western-blot and immuno-transmission electron microscopy. The antigenicity of RESA was evaluated by ELISA, using the carboxy-terminal repeat region as antigen. The assay's sensitivity and specificity were 78.2 and 94% respectively.es_ES
dc.description.sponsorshipThe study was funded by the Instituto de Salud Carlos III, Spanish Research Network of Tropical Diseases (RICET; RD06/0021/0000), Ministry of Science and Innovation of Spain.es_ES
dc.language.isoenges_ES
dc.publisherSpringer es_ES
dc.type.hasVersionVoRes_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/*
dc.subjectPlasmodium falciparumes_ES
dc.subjectMalariaes_ES
dc.subjectELISAes_ES
dc.subjectExpression libraryes_ES
dc.subjectElectron microscopyes_ES
dc.subject.meshAmino Acid Sequence es_ES
dc.subject.meshAntigens, Protozoan es_ES
dc.subject.meshBlotting, Western es_ES
dc.subject.meshCell Extracts es_ES
dc.subject.meshCloning, Molecular es_ES
dc.subject.meshComputational Biology es_ES
dc.subject.meshDNA, Complementary es_ES
dc.subject.meshGenetic Markers es_ES
dc.subject.meshHumans es_ES
dc.subject.meshMolecular Sequence Data es_ES
dc.subject.meshPlasmodium falciparum es_ES
dc.subject.meshProtein Transport es_ES
dc.subject.meshProtozoan Proteins es_ES
dc.subject.meshRecombinant Fusion Proteins es_ES
dc.subject.meshSequence Alignment es_ES
dc.subject.meshGenetic Variation es_ES
dc.titleMolecular cloning and characterisation of the RESA gene, a marker of genetic diversity of Plasmodium falciparumes_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución-NoComercial- 4.0 Internacional*
dc.identifier.pubmedID19816792es_ES
dc.format.volume37es_ES
dc.format.number6es_ES
dc.format.page2893-902es_ES
dc.identifier.doi10.1007/s11033-009-9849-zes_ES
dc.contributor.funderInstituto de Salud Carlos III 
dc.contributor.funderRETICS-Investigación colaborativa en Enfermedades Tropicales (RICET-ISCIII) (España) 
dc.description.peerreviewedes_ES
dc.identifier.e-issn1573-4978es_ES
dc.relation.publisherversionhttps://doi.org/10.1007/s11033-009-9849-zes_ES
dc.identifier.journalMolecular biology reportses_ES
dc.repisalud.centroISCIII::Centro Nacional de Medicina Tropicales_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.rights.accessRightsopen accesses_ES


Ficheros en el ítem

Acceso Abierto
Thumbnail

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem

Atribución-NoComercial- 4.0 Internacional
Este Item está sujeto a una licencia Creative Commons: Atribución-NoComercial- 4.0 Internacional