Mostrar el registro sencillo del ítem
dc.contributor.author | Lynch, Cian J | |
dc.contributor.author | Bernad, Raquel | |
dc.contributor.author | Calvo, Isabel | |
dc.contributor.author | Nóbrega-Pereira, Sandrina | |
dc.contributor.author | Ruiz, Sergio | |
dc.contributor.author | Ibarz, Nuria | |
dc.contributor.author | Martinez del Val, Ana | |
dc.contributor.author | Graña Castro, Osvaldo | |
dc.contributor.author | Gomez Lopez, Gonzalo | |
dc.contributor.author | Andrés-León, Eduardo | |
dc.contributor.author | Espinosa Angarica, Vladimir | |
dc.contributor.author | Del Sol, Antonio | |
dc.contributor.author | Ortega Jimenez, Sagrario | |
dc.contributor.author | Fernandez-Capetillo, Oscar | |
dc.contributor.author | Rojo, Enrique | |
dc.contributor.author | Muoz Peralta, Javier | |
dc.contributor.author | Serrano Marugan , Manuel | |
dc.date.accessioned | 2018-11-14T09:42:15Z | |
dc.date.available | 2018-11-14T09:42:15Z | |
dc.date.issued | 2018-01-09 | |
dc.identifier.citation | Cell Rep. 2018; 22(2): 396-410. | es_ES |
dc.identifier.issn | 22111247 | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/6584 | |
dc.description.abstract | The RNA polymerase II-associated protein 1 (RPAP1) is conserved across metazoa and required for stem cell differentiation in plants; however, very little is known about its mechanism of action or its role in mammalian cells. Here, we report that RPAP1 is essential for the expression of cell identity genes and for cell viability. Depletion of RPAP1 triggers cell de-differentiation, facilitates reprogramming toward pluripotency, and impairs differentiation. Mechanistically, we show that RPAP1 is essential for the interaction between RNA polymerase II (RNA Pol II) and Mediator, as well as for the recruitment of important regulators, such as the Mediator-specific RNA Pol II factor Gdown1 and the C-terminal domain (CTD) phosphatase RPAP2. In agreement, depletion of RPAP1 diminishes the loading of total and Ser5-phosphorylated RNA Pol II on many genes, with super-enhancer-driven genes among the most significantly downregulated. We conclude that Mediator/RPAP1/RNA Pol II is an ancient module, conserved from plants to mammals, critical for establishing and maintaining cell identity. | es_ES |
dc.description.sponsorship | We are grateful to Elisa Varela for assistance with morula and blastocyst fixa- tion. Work in the laboratory of M.S. is funded by the CNIO and the IRB and by grants from the Spanish Ministry of Economy co-funded by the European Regional Development Fund (ERDF) (SAF2013-48256-R), the European Research Co uncil (ERC-2014-AdG/66 9622), the Region al Government of Ma- drid co-funded by the Euro pean Social Fund (ReCaRe project), the Euro pean Union (RISK-IR project), the Botin Foundation and Banco Santander (Santander Universities Glo bal Division), the Ramon Areces Found ation, and the AXA Foundation. S.R. was funded by a contract from the Ramon y Cajal Program(RYC-2011-09242) and by the Spanish Ministry of Economy co- funded by the ERDF (SAF2013-49147- P and SAF2016-80874-P | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Mediator | es_ES |
dc.subject | RNA polymerase II | es_ES |
dc.subject | Cell identity | es_ES |
dc.subject | Differentiation | es_ES |
dc.subject | Enhancer | es_ES |
dc.subject | Interactome | es_ES |
dc.subject | Transcription | es_ES |
dc.title | The RNA Polymerase II Factor RPAP1 Is Critical for Mediator-Driven Transcription and Cell Identity | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 29320736 | es_ES |
dc.format.volume | 22 | es_ES |
dc.format.number | 2 | es_ES |
dc.format.page | 396-410 | es_ES |
dc.identifier.doi | 10.1016/j.celrep.2017.12.062 | es_ES |
dc.contributor.funder | Unión Europea. Comisión Europea. European Research Council (ERC) | |
dc.contributor.funder | Botín Foundation | |
dc.contributor.funder | Fundación Ramón Areces | |
dc.contributor.funder | Fundación AXA | |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | |
dc.description.peerreviewed | Sí | |
dc.identifier.e-issn | 2211-1247 | es_ES |
dc.relation.publisherversion | https://doi.org/10.1016/j.celrep.2017.12.062 | es_ES |
dc.identifier.journal | Cell reports | es_ES |
dc.repisalud.institucion | CNIO | es_ES |
dc.repisalud.orgCNIO | CNIO::Grupos de investigación | es_ES |
dc.repisalud.orgCNIO | CNIO::Grupos de investigación::Grupo de Supresión Tumoral | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/SAF2013-48256-R | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/SAF2013-49147-P | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/SAF2016-80874-P | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/H2020/669622 | es_ES |
dc.rights.accessRights | open access | es_ES |