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dc.contributor.authorLynch, Cian J
dc.contributor.authorBernad, Raquel
dc.contributor.authorCalvo, Isabel
dc.contributor.authorNóbrega-Pereira, Sandrina
dc.contributor.authorRuiz, Sergio
dc.contributor.authorIbarz, Nuria
dc.contributor.authorMartinez del Val, Ana 
dc.contributor.authorGraña Castro, Osvaldo 
dc.contributor.authorGomez Lopez, Gonzalo 
dc.contributor.authorAndrés-León, Eduardo
dc.contributor.authorEspinosa Angarica, Vladimir
dc.contributor.authorDel Sol, Antonio
dc.contributor.authorOrtega Jimenez, Sagrario 
dc.contributor.authorFernandez-Capetillo, Oscar 
dc.contributor.authorRojo, Enrique
dc.contributor.authorMuoz Peralta, Javier 
dc.contributor.authorSerrano Marugan , Manuel 
dc.date.accessioned2018-11-14T09:42:15Z
dc.date.available2018-11-14T09:42:15Z
dc.date.issued2018-01-09
dc.identifier.citationCell Rep. 2018; 22(2): 396-410.es_ES
dc.identifier.issn22111247es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/6584
dc.description.abstractThe RNA polymerase II-associated protein 1 (RPAP1) is conserved across metazoa and required for stem cell differentiation in plants; however, very little is known about its mechanism of action or its role in mammalian cells. Here, we report that RPAP1 is essential for the expression of cell identity genes and for cell viability. Depletion of RPAP1 triggers cell de-differentiation, facilitates reprogramming toward pluripotency, and impairs differentiation. Mechanistically, we show that RPAP1 is essential for the interaction between RNA polymerase II (RNA Pol II) and Mediator, as well as for the recruitment of important regulators, such as the Mediator-specific RNA Pol II factor Gdown1 and the C-terminal domain (CTD) phosphatase RPAP2. In agreement, depletion of RPAP1 diminishes the loading of total and Ser5-phosphorylated RNA Pol II on many genes, with super-enhancer-driven genes among the most significantly downregulated. We conclude that Mediator/RPAP1/RNA Pol II is an ancient module, conserved from plants to mammals, critical for establishing and maintaining cell identity.es_ES
dc.description.sponsorshipWe are grateful to Elisa Varela for assistance with morula and blastocyst fixa- tion. Work in the laboratory of M.S. is funded by the CNIO and the IRB and by grants from the Spanish Ministry of Economy co-funded by the European Regional Development Fund (ERDF) (SAF2013-48256-R), the European Research Co uncil (ERC-2014-AdG/66 9622), the Region al Government of Ma- drid co-funded by the Euro pean Social Fund (ReCaRe project), the Euro pean Union (RISK-IR project), the Botin Foundation and Banco Santander (Santander Universities Glo bal Division), the Ramon Areces Found ation, and the AXA Foundation. S.R. was funded by a contract from the Ramon y Cajal Program(RYC-2011-09242) and by the Spanish Ministry of Economy co- funded by the ERDF (SAF2013-49147- P and SAF2016-80874-Pes_ES
dc.language.isoenges_ES
dc.publisherElsevier es_ES
dc.type.hasVersionVoRes_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectMediatores_ES
dc.subjectRNA polymerase IIes_ES
dc.subjectCell identityes_ES
dc.subjectDifferentiationes_ES
dc.subjectEnhanceres_ES
dc.subjectInteractomees_ES
dc.subjectTranscriptiones_ES
dc.titleThe RNA Polymerase II Factor RPAP1 Is Critical for Mediator-Driven Transcription and Cell Identityes_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID29320736es_ES
dc.format.volume22es_ES
dc.format.number2es_ES
dc.format.page396-410es_ES
dc.identifier.doi10.1016/j.celrep.2017.12.062es_ES
dc.contributor.funderUnión Europea. Comisión Europea. European Research Council (ERC) 
dc.contributor.funderBotín Foundation 
dc.contributor.funderFundación Ramón Areces 
dc.contributor.funderFundación AXA 
dc.contributor.funderMinisterio de Economía y Competitividad (España) 
dc.description.peerreviewed
dc.identifier.e-issn2211-1247es_ES
dc.relation.publisherversionhttps://doi.org/10.1016/j.celrep.2017.12.062es_ES
dc.identifier.journalCell reportses_ES
dc.repisalud.institucionCNIOes_ES
dc.repisalud.orgCNIOCNIO::Grupos de investigaciónes_ES
dc.repisalud.orgCNIOCNIO::Grupos de investigación::Grupo de Supresión Tumorales_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2013-48256-Res_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2013-49147-Pes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2016-80874-Pes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/669622es_ES
dc.rights.accessRightsopen accesses_ES


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Este Item está sujeto a una licencia Creative Commons: Atribución 4.0 Internacional