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dc.contributor.authorCallejo, Maria
dc.contributor.authorMondejar-Parreno, Gema
dc.contributor.authorBarreira, Bianca
dc.contributor.authorIzquierdo-Garcia, Jose L. 
dc.contributor.authorMorales-Cano, Daniel
dc.contributor.authorEsquivel-Ruiz, Sergio
dc.contributor.authorMoreno, Laura
dc.contributor.authorCogolludo, Angel
dc.contributor.authorDuarte, Juan
dc.contributor.authorPerez-Vizcaino, Francisco
dc.date.accessioned2018-11-05T11:58:21Z
dc.date.available2018-11-05T11:58:21Z
dc.date.issued2018
dc.identifierISI:000436233600009
dc.identifier.citationSci Rep. 2018; 8(1):9681
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/20.500.12105/6575
dc.description.abstractWe have analysed whether pulmonary arterial hypertension (PAH) alters the rat faecal microbiota. Wistar rats were injected with the VEGF receptor antagonist SU5416 (20 mg/kg s.c.) and followed for 2 weeks kept in hypoxia (10\% O-2, PAH) or injected with vehicle and kept in normoxia (controls). Faecal samples were obtained and microbiome composition was determined by 165 rRNA gene sequencing and bioinformatic analysis. No effect of PAH on the global microbiome was found (alpha- or beta-diversity). However, PAH-exposed rats showed gut dysbiosis as indicated by a taxonomy-based analysis. Specifically, PAH rats had a three-fold increase in Firmicutes-to-Bacteroidetes ratio. Within the Firmicutes phylum, there were no large changes in the relative abundance of the bacterial families in PAH. Among Bacteroidetes, all families were less abundant in PAH. A clear separation was observed between the control and PAH clusters based on short chain fatty acid producing bacterial genera. Moreover, acetate was reduced in the serum of PAH rats. In conclusion, faecal microbiota composition is altered as a result of PAH. This misbalanced bacterial ecosystem might in turn play a pathophysiological role in PAH by altering the immunologic, hormonal and metabolic homeostasis.
dc.description.sponsorshipWe thank Dr. Ricardo Ramos from Parque Cientifico de Madrid for helpful advices and for revising and editing the methods section. This study is supported by grants from Mineco (SAF2014-55399-R, SAF2014-55523-R, SAF2016-77222 and SAF2017-84494-C2-1R), Instituto de Salud Carlos III (PI15/01100), with funds from the European Union (Fondo Europeo de Desarrollo Regional FEDER). M.C., G.M-P. and S.E-R. are funded by Universidad Complutense, Fondo de Garantia Juvenil (Comunidad de Madrid) and Ciberes grant with funds from Fundacion Contra la Hipertension Pulmonar, a FPU grant from Ministerio de Educacion, respectively. J.L.I.G is a CNIC IPP COFUND Fellow and has received funding from the People Programme (Marie Curie Actions) of the FP7/2007-2013 under REA grant agreement no 600396. The CNIC is supported by MEIC-AEI and the Pro CNIC Foundation, and is a Severo Ochoa Center of Excellence (MEIC award SEV-2015-0505).
dc.language.isoeng
dc.publisherNature Publishing Group 
dc.type.hasVersionVoR
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectSLEEP-APNEA
dc.subjectDISEASE
dc.subjectDYSBIOSIS
dc.subjectIMMUNITY
dc.subjectINFLAMMATION
dc.subjectINFECTION
dc.subjectBACTERIA
dc.subjectLUNG
dc.subjectCARCINOGENESIS
dc.subjectINHIBITION
dc.titlePulmonary Arterial Hypertension Affects the Rat Gut Microbiome
dc.typejournal article
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID29946072
dc.format.volume8
dc.identifier.doi10.1038/s41598-018-27682-w
dc.contributor.funderMinisterio de Economía y Competitividad (España) 
dc.contributor.funderInstituto de Salud Carlos III 
dc.contributor.funderUnión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) 
dc.contributor.funderComplutense University of Madrid (España) 
dc.contributor.funderFundación Contra la Hipertensión Pulmonar 
dc.contributor.funderMinisterio de Educación (España) 
dc.contributor.funderUnión Europea. Comisión Europea 
dc.contributor.funderAgencia Estatal de Investigación (España) 
dc.contributor.funderFundación ProCNIC 
dc.contributor.funderComunidad de Madrid (España) 
dc.description.peerreviewed
dc.relation.publisherversionhttps://doi.org/10.1038/s41598-018-27682-w
dc.identifier.journalScientific Reports
dc.repisalud.orgCNICCNIC::Unidades técnicas
dc.repisalud.institucionCNIC
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/600396/EUes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2014-55399-Res_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2014-55523-Res_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2016-77222es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2017-84494-C2-1Res_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/PI15/01100es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SEV-2015-0505es_ES
dc.rights.accessRightsopen accesses_ES


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