Publication: A novel nano-immunoassay method for quantification of proteins from CD138-purified myeloma cells: biological and clinical utility
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2018-05
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European Hematology Association (EHA)
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Abstract
Protein analysis in bone marrow samples from patients with multiple myeloma has been limited by the low concentration of proteins obtained after CD138+ cell selection. A novel approach based on capillary nano-immunoassay could make it possible to quantify dozens of proteins from each myeloma sample in an automated manner. Here we present a method for the accurate and robust quantification of the expression of multiple proteins extracted from CD138-purified multiple myeloma samples frozen in RLT Plus buffer, which is commonly used for nucleic acid preservation and isolation. Additionally, the biological and clinical value of this analysis for a panel of 12 proteins essential to the pathogenesis of multiple myeloma was evaluated in 63 patients with newly diagnosed multiple myeloma. The analysis of the prognostic impact of CRBN/Cereblon and IKZF1/Ikaros mRNA/protein showed that only the protein levels were able to predict progression-free survival of patients; mRNA levels were not associated with prognosis. Interestingly, high levels of Cereblon and Ikaros proteins were associated with longer progression-free survival only in patients who received immunomodulatory drugs and not in those treated with other drugs. In conclusion, the capillary nano-immunoassay platform provides a novel opportunity for automated quantification of the expression of more than 20 proteins in CD138+ primary multiple myeloma samples.
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This work was funded by a grant from the International
Myeloma Foundation's Black Swan Research Initiative
®and“Gerencia Regional de Salud, Junta de Castilla y León”
(BIO/SA35/14). WES™platform was acquired thanks to
INNOCAMPUS Program (CEI10-1-0010
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Haematologica. 2018; 103 (5) : 880 - 889.