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dc.contributor.authorGonzalez-Rosa, Juan Manuel 
dc.contributor.authorGuzman-Martinez, Gabriela 
dc.contributor.authorMarques, Ines Joao 
dc.contributor.authorSanchez-Iranzo, Hector 
dc.contributor.authorJimenez-Borreguero, Luis J. 
dc.contributor.authorMercader, Nadia 
dc.date.accessioned2017-12-01T07:37:28Z
dc.date.available2017-12-01T07:37:28Z
dc.date.issued2014
dc.identifierISI:000348845100084
dc.identifier.citationPLoS One. 2014; 9(12):e115604
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/20.500.12105/5530
dc.description.abstractAims: While zebrafish embryos are amenable to in vivo imaging, allowing the study of morphogenetic processes during development, intravital imaging of adults is hampered by their small size and loss of transparency. The use of adult zebrafish as a vertebrate model of cardiac disease and regeneration is increasing at high speed. It is therefore of great importance to establish appropriate and robust methods to measure cardiac function parameters. Methods and Results: Here we describe the use of 2D-echocardiography to study the fractional volume shortening and segmental wall motion of the ventricle. Our data show that 2D-echocardiography can be used to evaluate cardiac injury and also to study recovery of cardiac function. Interestingly, our results show that while global systolic function recovered following cardiac cryoinjury, ventricular wall motion was only partially restored. Conclusion: Cryoinjury leads to long-lasting impairment of cardiac contraction, partially mimicking the consequences of myocardial infarction in humans. Functional assessment of heart regeneration by echocardiography allows a deeper understanding of the mechanisms of cardiac regeneration and has the advantage of being easily transferable to other cardiovascular zebrafish disease models.
dc.description.sponsorshipFunding was from the Fundacion CNIC Carlos III, the Fundacion ProCNIC, the Spanish Ministry of Economy and Competitiveness (Tercel and BFU2011-25297 to N.M., FPU AP2008-00546 to J.M.G.-R. and FPU12/03007 to H.S-I.), the Community of Madrid (FIBROTEAM S2010/BMD-2321 to N.M), PIEF-GA-2012-330728 to I.J.M. and an ERC Starting grant 337703 - zebraHeart to N.M. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
dc.language.isoeng
dc.publisherPublic Library of Science (PLOS) 
dc.type.hasVersionVoR
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectHEART REGENERATION
dc.subjectADULT ZEBRAFISH
dc.subjectMYOCARDIAL-INFARCTION
dc.subjectCARDIAC REGENERATION
dc.subjectGENE
dc.subjectREPRODUCIBILITY
dc.subjectEXPRESSION
dc.subjectDISEASE
dc.titleUse of Echocardiography Reveals Reestablishment of Ventricular Pumping Efficiency and Partial Ventricular Wall Motion Recovery upon Ventricular Cryoinjury in the Zebrafish
dc.typejournal article
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID25532015
dc.format.volume9
dc.identifier.doi10.1371/journal.pone.0115604
dc.contributor.funderUnión Europea. Comisión Europea 
dc.contributor.funderFundación ProCNIC 
dc.contributor.funderMinisterio de Economía y Competitividad (España) 
dc.contributor.funderComunidad de Madrid (España) 
dc.contributor.funderUnión Europea. Comisión Europea. European Research Council (ERC) 
dc.description.peerreviewed
dc.relation.publisherversionhttps://doi.org/10.1371/journal.pone.0115604
dc.identifier.journalPLoS One
dc.repisalud.orgCNICCNIC::Grupos de investigación::Desarrollo del Epicardio y su Papel en la Regeneración
dc.repisalud.institucionCNIC
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/337703/EUes_ES
dc.rights.accessRightsopen accesses_ES


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Atribución 4.0 Internacional
Este Item está sujeto a una licencia Creative Commons: Atribución 4.0 Internacional