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dc.contributor.authorSoto, Manuel
dc.contributor.authorCorvo, Laura
dc.contributor.authorGarde, Esther
dc.contributor.authorRamirez, Laura
dc.contributor.authorIniesta, Virginia
dc.contributor.authorBonay, Pedro
dc.contributor.authorGomez-Nieto, Carlos
dc.contributor.authorGonzalez, Victor M.
dc.contributor.authorElena Martin, M.
dc.contributor.authorAlonso, Carlos
dc.contributor.authorCoelho, Eduardo A. F.
dc.contributor.authorBarral, Aldina
dc.contributor.authorBarral-Netto, Manoel
dc.contributor.authorIborra, Salvador 
dc.date.accessioned2017-11-27T13:49:52Z
dc.date.available2017-11-27T13:49:52Z
dc.date.issued2015
dc.identifierISI:000355303600021
dc.identifier.citationPLoS Negl Trop Dis. 2015; 9(5):e0003751
dc.identifier.issn1935-2735
dc.identifier.urihttp://hdl.handle.net/20.500.12105/5406
dc.description.abstractBackground Highly conserved intracellular proteins from Leishmania have been described as antigens in natural and experimental infected mammals. The present study aimed to evaluate the antigenicity and prophylactic properties of the Leishmania infantum Poly (A) binding proteins (LiPABPs). Methodology/Principal Findings Three different members of the LiPABP family have been described. Recombinant tools based on these proteins were constructed: recombinant proteins and DNA vaccines. The three recombinant proteins were employed for coating ELISA plates. Sera from human and canine patients of visceral leishmaniasis and human patients of mucosal leishmaniasis recognized the three LiPABPs. In addition, the protective efficacy of a DNA vaccine based on the combination of the three Leishmania PABPs has been tested in a model of progressive murine leishmaniasis: BALB/c mice infected with Leishmania major. The induction of a Th1-like response against the LiPABP family by genetic vaccination was able to down-regulate the IL-10 predominant responses elicited by parasite LiPABPs after infection in this murine model. This modulation resulted in a partial protection against L. major infection. LiPABP vaccinated mice showed a reduction on the pathology that was accompanied by a decrease in parasite burdens, in antibody titers against Leishmania antigens and in the IL-4 and IL-10 parasite-specific mediated responses in comparison to control mice groups immunized with saline or with the non-recombinant plasmid. Conclusion/Significance The results presented here demonstrate for the first time the prophylactic properties of a new family of Leishmania antigenic intracellular proteins, the LiPABPs. The redirection of the immune response elicited against the LiPABP family (from IL-10 towards IFN-gamma mediated responses) by genetic vaccination was able to induce a partial protection against the development of the disease in a highly susceptible murine model of leishmaniasis.
dc.description.sponsorshipThe study was supported in Spain by grants from Ministerio de Ciencia e Innovacion FIS PI11/00095 and FISPI14/00366 from the Instituto de Salud Carlos III within the Network of Tropical Diseases Research (VI P I+D+I 2008-2011, ISCIII -Subdireccion General de Redes y Centros de Investigacion Cooperativa (RD12/0018/0009)). This work was also supported in Brazil by a grant from CNPq (Ciencia sem Fronteiras-PVE 300174/2014-4). A CBMSO institutional grant from Fundacion Ramon Areces is also acknowledged. EAFC is a grant recipient of CNPq. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
dc.language.isoeng
dc.publisherPUBLIC LIBRARY SCIENCE
dc.relation.isversionofPublisher's version
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectCANINE VISCERAL LEISHMANIASIS
dc.subjectPRIME-BOOST VACCINATION
dc.subjectCUTANEOUS LEISHMANIASIS
dc.subjectT-CELL
dc.subjectIMMUNE-RESPONSES
dc.subjectVIANNIA BRAZILIENSIS
dc.subjectEXPRESSION CLONING
dc.subjectCONFERS PROTECTION
dc.subjectINCLUSION-BODIES
dc.subjectHISTONE H2B
dc.titleCoadministration of the Three Antigenic Leishmania infantum Poly (A) Binding Proteins as a DNA Vaccine Induces Protection against Leishmania major Infection in BALB/c Mice
dc.typeArtículo
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID25955652
dc.format.volume9
dc.identifier.doi10.1371/journal.pntd.0003751
dc.contributor.funderMinisterio de Ciencia e Innovación (España)
dc.contributor.funderRed de Investigación Cooperativa en Enfermedades Tropicales (España)
dc.contributor.funderInstituto de Salud Carlos III - ISCIII
dc.contributor.funderFundacion Ramon Areces
dc.contributor.funderConselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil)
dc.description.peerreviewed
dc.relation.publisherversionhttps://doi.org/10.1371/journal.pntd.0003751
dc.identifier.journalPLoS Neglected Tropical Diseases
dc.repisalud.orgCNICCNIC::Grupos de investigación::Inmunobiología
dc.repisalud.institucionCNIC
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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