Please use this identifier to cite or link to this item:http://hdl.handle.net/20.500.12105/5406
Title
Coadministration of the Three Antigenic Leishmania infantum Poly (A)
Binding Proteins as a DNA Vaccine Induces Protection against Leishmania
major Infection in BALB/c Mice
Author(s)
Date issued
2015
Citation
PLoS Negl Trop Dis. 2015; 9(5):e0003751
Language
Inglés
Abstract
Background
Highly conserved intracellular proteins from Leishmania have been
described as antigens in natural and experimental infected mammals. The
present study aimed to evaluate the antigenicity and prophylactic
properties of the Leishmania infantum Poly (A) binding proteins
(LiPABPs).
Methodology/Principal Findings
Three different members of the LiPABP family have been described.
Recombinant tools based on these proteins were constructed: recombinant
proteins and DNA vaccines. The three recombinant proteins were employed
for coating ELISA plates. Sera from human and canine patients of
visceral leishmaniasis and human patients of mucosal leishmaniasis
recognized the three LiPABPs. In addition, the protective efficacy of a
DNA vaccine based on the combination of the three Leishmania PABPs has
been tested in a model of progressive murine leishmaniasis: BALB/c mice
infected with Leishmania major. The induction of a Th1-like response
against the LiPABP family by genetic vaccination was able to
down-regulate the IL-10 predominant responses elicited by parasite
LiPABPs after infection in this murine model. This modulation resulted
in a partial protection against L. major infection. LiPABP vaccinated
mice showed a reduction on the pathology that was accompanied by a
decrease in parasite burdens, in antibody titers against Leishmania
antigens and in the IL-4 and IL-10 parasite-specific mediated responses
in comparison to control mice groups immunized with saline or with the
non-recombinant plasmid.
Conclusion/Significance
The results presented here demonstrate for the first time the
prophylactic properties of a new family of Leishmania antigenic
intracellular proteins, the LiPABPs. The redirection of the immune
response elicited against the LiPABP family (from IL-10 towards
IFN-gamma mediated responses) by genetic vaccination was able to induce
a partial protection against the development of the disease in a highly
susceptible murine model of leishmaniasis.
Subject
CANINE VISCERAL LEISHMANIASIS | PRIME-BOOST VACCINATION | CUTANEOUS
LEISHMANIASIS | T-CELL | IMMUNE-RESPONSES | VIANNIA BRAZILIENSIS | EXPRESSION CLONING | CONFERS PROTECTION | INCLUSION-BODIES | HISTONE H2B
Online version
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