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dc.contributor.authorTorres Ruiz, Raul 
dc.contributor.authorRodriguez Perales, Sandra 
dc.date.accessioned2017-11-27T13:49:50Z
dc.date.available2017-11-27T13:49:50Z
dc.date.issued2015
dc.identifierISI:000364541000112
dc.identifier.citationInt J Mol Sci. 2015; 16(9):22151-68
dc.identifier.issn1422-0067
dc.identifier.urihttp://hdl.handle.net/20.500.12105/5393
dc.description.abstractThe cancer-modelling field is now experiencing a conversion with the recent emergence of the RNA-programmable CRISPR-Cas9 system, a flexible methodology to produce essentially any desired modification in the genome. Cancer is a multistep process that involves many genetic mutations and other genome rearrangements. Despite their importance, it is difficult to recapitulate the degree of genetic complexity found in patient tumors. The CRISPR-Cas9 system for genome editing has been proven as a robust technology that makes it possible to generate cellular and animal models that recapitulate those cooperative alterations rapidly and at low cost. In this review, we will discuss the innovative applications of the CRISPR-Cas9 system to generate new models, providing a new way to interrogate the development and progression of cancers.
dc.description.sponsorshipThis work was supported by FIS project no PI14/01884 to Sandra Rodriguez-Perales from the Spanish plan nacional I+d+, Instituto de Salud Carlos III FEDER. The funders had no role in the study design, data collection and analysis, decision to publish nor preparation of the manuscript.
dc.language.isoeng
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI) 
dc.relation.isversionofPublisher's version
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectCRISPR-Cas9 system
dc.subjectcancer modelling
dc.subjectgene mutations
dc.subjectrearrangements
dc.subjectgenome engineering
dc.subjectZINC-FINGER NUCLEASES
dc.subjectOFF-TARGET MUTATIONS
dc.subjectONE-STEP GENERATION
dc.subjectIN-VIVO
dc.subjectHUMAN-CELLS
dc.subjectHUMAN GENOME
dc.subjectCHROMOSOMAL REARRANGEMENTS
dc.subjectCAS9 RIBONUCLEOPROTEINS
dc.subjectTUMOR-SUPPRESSOR
dc.subjectMAMMALIAN-CELLS
dc.titleCRISPR-Cas9: A Revolutionary Tool for Cancer Modelling
dc.typeArtículo
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID26389881
dc.format.volume16
dc.format.page22151-22168
dc.identifier.doi10.3390/ijms160922151
dc.contributor.funderInstituto de Salud Carlos III - ISCIII
dc.description.peerreviewed
dc.relation.publisherversionhttps://doi.org/10.3390/ijms160922151
dc.identifier.journalInternational Journal of Molecular Sciences
dc.repisalud.orgCNICCNIC::Unidades técnicas::Vectores Virales
dc.repisalud.institucionCNIC
dc.repisalud.institucionCNIO
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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