Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/5393
Título
CRISPR-Cas9: A Revolutionary Tool for Cancer Modelling
Autor(es)
Torres-Ruiz, Raul CNIC | Rodriguez Perales, Sandra CNIO
Fecha de publicación
2015
Cita
Int J Mol Sci. 2015; 16(9):22151-68
Idioma
Inglés
Tipo de documento
journal article
Resumen
The cancer-modelling field is now experiencing a conversion with the
recent emergence of the RNA-programmable CRISPR-Cas9 system, a flexible
methodology to produce essentially any desired modification in the
genome. Cancer is a multistep process that involves many genetic
mutations and other genome rearrangements. Despite their importance, it
is difficult to recapitulate the degree of genetic complexity found in
patient tumors. The CRISPR-Cas9 system for genome editing has been
proven as a robust technology that makes it possible to generate
cellular and animal models that recapitulate those cooperative
alterations rapidly and at low cost. In this review, we will discuss the
innovative applications of the CRISPR-Cas9 system to generate new
models, providing a new way to interrogate the development and
progression of cancers.
Palabras clave
CRISPR-Cas9 system | Cancer modelling | Gene mutations | Rearrangements | Genome engineering | ZINC-FINGER NUCLEASES | OFF-TARGET MUTATIONS | ONE-STEP GENERATION | IN-VIVO | HUMAN-CELLS | HUMAN GENOME | CHROMOSOMAL REARRANGEMENTS | CAS9 RIBONUCLEOPROTEINS | TUMOR-SUPPRESSOR | MAMMALIAN-CELLS
Versión en línea
DOI
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