Please use this identifier to cite or link to this item:http://hdl.handle.net/20.500.12105/5242
Membrane Blebbing Is Required for Mesenchymal Precursor Migration
de Lucas, Beatriz CNIC | Bernal, Aurora CNIC | Perez, Laura M. CNIC | San Martin, Nuria CNIC | Galvez, Beatriz G. CNIC
PLoS One. 2016; 11(3):e0150004
Mesenchymal precursors (MPs) present some advantageous features, such as differentiation and migration, which make them promising candidates for cell therapy. A better understanding of MP migration characteristics would aid the development of cell delivery protocols. Traditionally, cell migration is thought to occur only through the formation of lamellipodia. More recently, contractility-driven bleb formation has emerged as an alternative mechanism of motility. Here we report that MPs derived from different tissues present spontaneously dynamic cytoplasmic projections in sub-confluent culture, which appear as a combination of lamellipodia with blebs in the leading edge. Upon initial seeding, however, only bleb structures could be observed. Immunofluorescence revealed the presence of pERM, RhoA and F-actin during the blebbing process. Results from migration assays in the presence of blebbistatin, a myosin II inhibitor, showed that bleb formation correlated with migratory capacity, suggesting a functional role for blebs in migration. Bleb formation might be a useful mechanism to improve cell migration in cellular therapy protocols.
STEM-CELLS | CARDIAC MESOANGIOBLASTS | AMEBOID MIGRATION | SKELETAL-MUSCLE | INVASION | BLEBS | CONTRACTILITY | LAMELLIPODIA | BIOMECHANICS | MECHANICS
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